http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
1 online resource (x, 473 pages) : illustrations.
Natural Products Isolation provides a comprehensive introduction to techniques for the extraction and purification of natural products from all biological sources. The book opens with an introduction to separations and chromatography and discusses the approach to an isolation. Experienced experimentalists describe a wide array of methods for isolation of both known and unknown natural products, including initial extraction, open column chromatography, HPLC, countercurrent and planar chromatography, SFE, and crystallisation. Later chapters address specific issues in working with plants, marine organisms, water-soluble compounds, as well as scale-up, dereplication and follow-up of a natural product lead. For the less experienced, these chapters provide background information and hands-on advice - using real examples - about how to approach an extraction in general, when and how to apply a particular technique and how to modify conditions according to the nature of the compound being isolated. Geared to scientists with little experience of natural products extraction, but offering even skilled researchers valuable advice and insight, Natural Products Isolation lays the foundation for the potential extractor to isolate natural substances efficiently. Its methods and guidance will almost certainly play a major role in today's natural product discovery and development.
How to approach the isolation of a natural product / Richard J.P. Cannell -- Initial extraction and product capture / F. Patrick Gailliot -- Supercritical fluid methods / Ed Venkat and Srinivas Kothandaraman -- Isolation by low-pressure column chromatography / Gino M. Salituro and Claude Dufresne -- Isolation by ion-exchange methods / Claude Dufresne -- Isolation by preparative HPLC / Paul Stead -- Isolation by planar chromatography / Simon Gibbons and Alexander I. Gray -- Separation by high-speed countercurrent chromatography / James McAlpine -- Crystallization and final stages of purification / Norman Shankland, Alastair J. Florence, and Richard J.P. Cannell -- Dereplication and partial identification of natural products / Frank VanMiddlesworth and Richard J.P. Cannell -- Purification of water-soluble natural products / Yuzuru Shimizu -- Special problems with the extraction of plants / Gloria L. Silva, Ik-Soo Lee, A. Douglas Kinghorn -- Isolation of marine natural products / Amy E. Wright -- Scale-up of natural products isolation / Michael S. Verrall and Stephen R.C. Warr -- Follow-up of natural product isolation / Richard J.P. Cannell.
Includes bibliographical references and index.