rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Principles and Technical Aspects of PCR Amplification
  name:
    name part: Pelt-Verkuil, Elizabeth van.
    role:
      role term: creator
    role:
      role term: author.
  name:
    name part: Belkum, Alex van.
    role:
      role term: author.
  name:
    name part: Hays, John P.
    role:
      role term: author.
  name:
    name part: SpringerLink (Online service)
  type of resource: text
  origin info:
    place:
      place term: ne
    date issued: 2008
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: electronic
    form: electronic resource
    reformatting quality: access
    form: remote
    extent: XII, 330 p. online resource.
  abstract: Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than 15 years ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described, with many of these adaptations and variations currently being used in clinical, diagnostic and academic laboratories across the world. Further, these techniques are being applied at the diagnostic level (e.g. as high throughput testing methodologies to detect minimum residual disease, the presence/absence of specific pathogens etc), as well as to increase our understanding of fundamental disease processes. Frequently, PCR technicians and specialists limit their understanding of PCR to one particular methodology. However, this approach limits their appreciation of the range of versatile PCR techniques currently available, techniques that may be applicable and indeed more suitable to their own laboratory situation. This manual aims to provide the reader with a guide to the standard PCR technique and its many available modifications, with particular emphasis on the role of PCR techniques in the diagnostic laboratory (the central theme of this manual). Further, many important technical issues have been addressed, including types of PCR template material, PCR optimization, the analysis of PCR products, quality control and quality assurance, variants and adaptations of the standard PCR protocol, quantitative PCR and in situ PCR. The reader of this manual will be excellently informed about the fundamental principles of PCR and the true potential of PCR within clinical laboratory practice.
  table of contents: The Polymerase Chain Reaction -- A Brief Comparison Between In Vivo DNA Replication and In Vitro PCR Amplification -- The PCR in Practice -- The Different Types and Varieties of Nucleic Acid Target Molecules -- PCR Primers -- Deoxynucleotide Triphosphates and Buffer Components -- Taq and Other Thermostable DNA Polymerases -- Important Considerations for Typical, Quantitative and Real-Time PCR Protocols -- Analysis of PCR Amplification Products -- Ensuring PCR Quality – Laboratory Organisation, PCR Optimization and Controls -- Ensuring PCR Quality – Quality Criteria and Quality Assurance -- Variants and Adaptations of the Standard PCR Protocol -- In Situ PCR Amplification (ISA) – Major Considerations, Sample Processing and Applications.
  note: by Elizabeth van Pelt-Verkuil, Alex van Belkum, John P. Hays.
  subject:
    topic: Life sciences
  subject:
    topic: Biochemistry
  subject:
    topic: Cell biology
  subject:
    topic: Biology
    topic: Technique
  subject:
    topic: Life Sciences
  subject:
    topic: Life Sciences, general
  subject:
    topic: Biomedicine general
  subject:
    topic: Biochemistry, general
  subject:
    topic: Cell Biology
  subject:
    topic: Biological Techniques
  classification: QH301-705
  classification: 570
  related item:
    title info:
      title: Springer eBooks
  identifier: 9781402062414
  identifier: http://dx.doi.org/10.1007/978-1-4020-6241-4
  record info:
    record creation date: 100301
    record change date: 20151125141729.0
    record identifier: 978-1-4020-6241-4