rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Application of Selected Reaction Monitoring to Highly Multiplexed Targeted Quantitative Proteomics
    sub title: A Replacement for Western Blot Analysis
  name:
    name part: Kinter, Michael.
    role:
      role term: creator
    role:
      role term: author.
  name:
    name part: Kinter, Caroline S.
    role:
      role term: author.
  name:
    name part: SpringerLink (Online service)
  type of resource: text
  origin info:
    place:
      place term: xxu
    date issued: 2013
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: electronic
    form: electronic resource
    reformatting quality: access
    form: remote
    extent: XIII, 65 p. 14 illus., 11 illus. in color. online resource.
  abstract: A key experiment in biomedical research is monitoring the expression of different proteins in order to detect changes that occur in biological systems under different experimental conditions. The method that is most widely used is the Western blot analysis. While Western blot is a workhorse in laboratories studying protein expression and has several advantages, it also has a number of significant limitations. In particular, the method is semi-quantitative with limited dynamic range. Western blot focuses on a single protein per sample with only a small number of representative samples analyzed in an experiment. New quantitative tools have been needed for some time to at least supplement, & possibly replace, the Western blot. Mass spectrometric methods have begun to compete with Western blot for routine quantitative analyses of proteins. One of these methods is based on the tandem mass spectrometry technique of selected reaction monitoring (SRM), which is also called multiple reaction monitoring (MRM). Selected reaction monitoring is actually an older tandem mass spectrometry technique, first described in the late 70s, that is widely utilized in the quantitative analysis of small molecules like drugs & metabolites. The use of selected reaction monitoring for the quantitative analysis of proteins has a number of advantages. Most importantly, it is fundamentally quantitative with a wide dynamic range. The output of the analysis is a numerical result that can range over several orders of magnitude. Other advantages include sufficient specificity & sensitivity to detect low abundance proteins in complex mixtures. Finally, selected reaction monitoring can be multiplexed to allow the quantitative analysis of relatively large numbers of proteins in a single sample in a single experiment. This Brief will explain both the theoretical & experimental details of the selected reaction monitoring experiment as it is applied to proteins.
  table of contents: The use of mass spectrometry for highly selective detection -- overview of how the selected reaction monitoring experiment works -- Designing a selected reaction monitoring method for a protein -- Example analyses include: sample processing, sample analysis, data processing -- Future Directions.
  note: by Michael Kinter, Caroline S. Kinter.
  subject:
    topic: Medicine
  subject:
    topic: Mass spectrometry
  subject:
    topic: Biochemistry
  subject:
    topic: Proteomics
  subject:
    topic: Biomedicine
  subject:
    topic: Biomedicine general
  subject:
    topic: Proteomics
  subject:
    topic: Mass Spectrometry
  subject:
    topic: Biochemistry, general
  classification: R-RZ
  classification: 610
  related item:
    title info:
      title: Springer eBooks
  related item:
    title info:
      title: SpringerBriefs in Systems Biology
  identifier: 9781461486664
  identifier: http://dx.doi.org/10.1007/978-1-4614-8666-4
  record info:
    record creation date: 130917
    record change date: 20130921042258.0
    record identifier: 978-1-4614-8666-4