rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Current Laboratory Methods in Neuroscience Research
  name:
    name part: Xiong, Huangui.
    role:
      role term: editor.
  name:
    name part: Gendelman, Howard E.
    role:
      role term: editor.
  name:
    name part: SpringerLink (Online service)
  type of resource: text
  origin info:
    place:
      place term: xxu
    date issued: 2014
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: electronic
    form: electronic resource
    reformatting quality: access
    form: remote
    extent: XIII, 585 p. 189 illus., 122 illus. in color. online resource.
  abstract: Current Laboratory Methods in Neuroscience Research is a research manual for both students and seasoned researchers. It focuses on commonly-used techniques employed in neuroscience research, presented in a simple, step-by-step manner for laboratory use. The manual also offers a “blueprint” for bench-to-bedside research designed to facilitate multidisciplinary neuroscience pursuits. Sections include coverage of neurohistological techniques, in vitro preparations, leukocyte isolation and application in neuroscience, standard laboratory nucleic acid and protein detections, nanomedicine, bioimaging, neuroelectrophysiology, immunohistochemistry and autoradiography, analysis of gene expression, and animal models.
  table of contents: Section 1: Neurohistological techniques -- 1. Brain tissue preparation, sectioning and staining -- 2. Brain stereotactic injections -- 3. Tract tracing at light and electron microscopy -- 4. Stereological analysis of neurological tissues -- Section 2: In vitro preparations -- 5. Preparation and use of rodent hippocampal brain slices -- 6. Single-Cell Neuronal Dissociation For Electrophysiological Studies -- 7. Isolation and Culture of Human Neurons, Microglia and Astrocytes -- 8. Isolation and Culture of Neural Stem/progenitor Cells -- 9. Isolation of mitochondria from brain tissue and cells -- 10. Isolation of synaptosomes from archived brain tissues -- 11. Xenopus oocytes isolation and microinjection -- Section 3: Leukocyte isolation and application in neuroscience -- 12. Centrifugal elutriation for studies of neuroimmunity -- 13. Flow cytometry in neuroscience research -- Section 4: Standard Laboratory Nucleic Acid and Protein Detections -- 14. Western Blotting Technique in Biomedical Research -- 15. Polymerase chain reaction (PCR) and Real-time RT PCR -- Section 5: Nanomedicine -- 16. Nanoformulations -- 17. Neuronanomedicine -- Section 6: Bioimaging -- 18. Confocal imaging of nerve cells -- 19. Magnetic resonance imaging (MRI) -- 20. X-Ray, Positron Emission, and Single Photon Emission Tomographic Bioimaging -- 21. Non-Invasive Neurophysiological Imaging with Magnetoencephalography -- 22. Multiphoton Brain Imaging -- Section 7: Neuroelectrophysiology -- 23. Techniques for Extracellular Recordings -- 24. “Blind” patch-clamp recordings from rodent hippocampal slices -- 25. Whole-cell patch clamp recordings -- Section 8: Immunohistochemistry and autoradiography -- 26. Analysis of Receptor Binding And Quantitative Autoradiography -- 27. Techniques in Immunohistochemistry and immunocytochemistry -- Section 9: Analysis of Gene Expression -- 28. Protein identification by mass spectrometry: Proteomics -- 29. DNA Microarrays as a Tool for Neurosciences Research -- 30. M…
  note: edited by Huangui Xiong, Howard E. Gendelman.
  subject:
    topic: Medicine
  subject:
    topic: Neurosciences
  subject:
    topic: Neurology
  subject:
    topic: Neurobiology
  subject:
    topic: Biomedicine
  subject:
    topic: Neurosciences
  subject:
    topic: Neurology
  subject:
    topic: Neurobiology
  classification: RC321-580
  classification: 612.8
  related item:
    title info:
      title: Springer eBooks
  identifier: 9781461487944
  identifier: http://dx.doi.org/10.1007/978-1-4614-8794-4
  record info:
    record creation date: 131104
    record change date: 20150519183947.0
    record identifier: 978-1-4614-8794-4