rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: How Free Cationic Polymer Chains Promote Gene Transfection
  name:
    name part: Yanan, Yue.
    role:
      role term: creator
    role:
      role term: author.
  name:
    name part: SpringerLink (Online service)
  type of resource: text
  origin info:
    place:
      place term: gw
    date issued: 2013
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: electronic
    form: electronic resource
    reformatting quality: access
    form: remote
    extent: XVIII, 94 p. 45 illus., 38 illus. in color. online resource.
  abstract: In this PhD thesis, Yue Yanan addresses a long-overlooked and critical question in the development of non-viral vectors for gene delivery. The author determines that those uncomplexed and cationic polymer chains free in the solution mixture of polymer and DNA facilitate and promote gene transfection. Furthermore, by using a combination of synthetic chemistry, polymer physics and molecular biology, Yue confirms that it is those cationic polymer chains free in the solution mixture, rather than those bound to DNA chains, that play a decisive role in intracellular trafficking. Instead of the previously proposed and widely accepted “proton sponge” model, the author's group propose a new hypothesis based on the results of several well-designed and decisive experiments. These results show that free polycationic chains with a length of more than ~10 nm are able to partially block the fusion between different endocytic vesicles, including the endocytic-vesicle-to-endolysosome pathway. This thesis is highly original and its results greatly deepen our understanding of polymer-mediated gene transfection. More importantly, it provides new insights into the rational design of next-generation superior polymeric gene-delivery vectors.
  table of contents: Introduction and background -- Revisiting complexation between DNA and polyethylenimine: the effect of uncomplexed chains free in the solution mixture on gene transfection -- Revisiting complexation between DNA and polyethylenimine: the effect of length of free polycationic chains on gene transfection -- Quantitative comparison of endocytosis and intracellular trafficking of DNA/polymer complexes in the absence/presence of free polycationic chains.
  note: by Yue Yanan.
  subject:
    topic: Chemistry
  subject:
    topic: Organic chemistry
  subject:
    topic: Polymers
  subject:
    topic: Nucleic acids
  subject:
    topic: Chemistry
  subject:
    topic: Polymer Sciences
  subject:
    topic: Organic Chemistry
  subject:
    topic: Nucleic Acid Chemistry
  classification: QD380-388
  classification: 541.2254
  related item:
    title info:
      title: Springer eBooks
  related item:
    title info:
      title: Springer Theses, Recognizing Outstanding Ph.D. Research
  identifier: 9783319003368
  identifier: http://dx.doi.org/10.1007/978-3-319-00336-8
  record info:
    record creation date: 130511
    record change date: 20151103122027.0
    record identifier: 978-3-319-00336-8