rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Purification of Peptides in High-Complexity Arrays
    sub title: A New Method for the Specific Surface Exchange and Purification of Entire Peptide Libraries
  name:
    name part: Schirwitz, Christopher.
    role:
      role term: creator
    role:
      role term: author.
  name:
    name part: SpringerLink (Online service)
  type of resource: text
  origin info:
    place:
      place term: gw
    date issued: 2013
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: electronic
    form: electronic resource
    reformatting quality: access
    form: remote
    extent: XIII, 132 p. 59 illus., 53 illus. in color. online resource.
  abstract: Christopher Schirwitz's thesis focuses on improving the quality of in situ synthesized high-complexity peptide micro arrays. Micro arrays containing proteins or small protein fragments in the form of peptides have become of great interest in proteomic research. With the help of these microarrays a large number of potential target molecules can be screened for interaction with a probe in a short timeframe. However, protein and peptide micro arrays are still lagging behind oligonucleotide arrays in terms of density, quality and manufacturing costs. A new approach developed at the German Cancer Research Center (DKFZ) has improved the synthesis of high-density peptide arrays. The current technology is capable of producing arrays with up to 40,000 different peptides per square cm by means of micro particle-based solid phase peptide synthesis. However, in situ synthesis approaches bear a conceptual disadvantage: The quality of the peptides is dependent on the efficiency of the synthesis so that peptide fragments are present in the resulting array among the desired full-length peptides. In peptide-protein interaction studies such peptide fragments. The central achievement of this thesis is the development of a new method allowing for the fast one-step purification of entire arrays without loss of resolution or spatial information. Christopher Schirwitz's work has resulted in a number of publications in high ranking journals.
  table of contents: Introduction -- Concept & State-of-the-Art -- Results & Discussion -- Conclusion -- Materials & Methods.
  note: by Christopher Schirwitz.
  subject:
    topic: Chemistry
  subject:
    topic: Bioorganic chemistry
  subject:
    topic: Physical chemistry
  subject:
    topic: Proteins
  subject:
    topic: Chemistry
  subject:
    topic: Bioorganic Chemistry
  subject:
    topic: Protein Science
  subject:
    topic: Physical Chemistry
  classification: QD241-441
  classification: 547
  related item:
    title info:
      title: Springer eBooks
  related item:
    title info:
      title: Springer Theses, Recognizing Outstanding Ph.D. Research
  identifier: 9783319008073
  identifier: http://dx.doi.org/10.1007/978-3-319-00807-3
  record info:
    record creation date: 130808
    record change date: 20151204145438.0
    record identifier: 978-3-319-00807-3