rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Membrane Proteins in Aqueous Solutions
    sub title: From Detergents to Amphipols
  name:
    name part: Popot, Jean-Luc.
    role:
      role term: creator
    role:
      role term: author.
    role:
      role term: aut
    role:
      role term: http://id.loc.gov/vocabulary/relators/aut
  name:
    name part: SpringerLink (Online service)
  type of resource: text
  origin info:
    place:
      place term: gw
    date issued: 2018
    edition: 1st ed. 2018.
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: electronic
    form: electronic resource
    reformatting quality: access
    form: remote
    extent: XXVII, 708 p. 437 illus., 266 illus. in color. online resource.
  abstract: Membrane proteins represent about one third of the proteins encoded in a cell's genome, and, because of their key physiological roles, more than half of drug targets. Detergents are traditionally used to extract proteins from membranes in order to make them amenable to the tools of biochemistry and biophysics. However, detergent-solubilized proteins are generally unstable. This has led to the development of alternative, non-conventional surfactants, such as bicelles, nanodiscs, amphipathic peptides, fluorinated surfactants, and specially designed amphipathic polymers called 'amphipols'. These novel tools, mainly developed over the past 20 years, are revolutionizing handling membrane proteins in vitro for basic and applied research, as well as for such biomedical applications as drug screening or vaccination. This book, written by a specialist of membrane proteins and one of the creators of amphipols, describes the properties and uses of these novel molecules. It opens with general introductions on membrane proteins and their natural environment, detergents, the current status of membrane protein in vitro studies, a broad panorama of non-conventional surfactants and a discussion of their respective advantages and limitations, and the preparation and properties of amphipols and membrane protein/amphipol complexes. Topical chapters cover in vitro folding, cell-free synthesis and stabilization of membrane proteins, and such biophysical and biochemical applications as electron microscopy, Xray diffraction, NMR, optical spectroscopy, mass spectrometry, the whole range of solutions studies, proteomics, and such practical applications as membrane protein immobilization and drug screening and the use of amphipols in vivo for vaccination and drug delivery. Each topical chapter is introduced with a concise, up-to-date overview of how membrane proteins are currently studied using each individual technique, before offering an exhaustive coverage and in-depth discussion of the co…
  table of contents: From the Contents: Membrane proteins: functions, structures, environments -- Taking membrane proteins out of their natural environment -- Alternatives to detergents for handling membrane proteins in aqueous solutions -- Chemical structure and physical-chemical properties of amphipols.
  note: by Jean-Luc Popot.
  subject:
    topic: Biophysics
  subject:
    topic: Biological physics
  subject:
    topic: Medicinal chemistry
  subject:
    topic: Proteins
  subject:
    topic: Pharmaceutical technology
  subject:
    topic: Polymers
  subject:
    topic: Physical chemistry
  subject:
    topic: Biological and Medical Physics, Biophysics
  subject:
    topic: Medicinal Chemistry
  subject:
    topic: Protein Science
  subject:
    topic: Pharmaceutical Sciences/Technology
  subject:
    topic: Polymer Sciences
  subject:
    topic: Physical Chemistry
  classification: QH505
  classification: 571.4
  related item:
    title info:
      title: Springer eBooks
  related item:
    title info:
      title: Biological and Medical Physics, Biomedical Engineering
  identifier: 9783319731483
  identifier: https://doi.org/10.1007/978-3-319-73148-3
  record info:
    record creation date: 180608
    record change date: 20191026081820.0
    record identifier: 978-3-319-73148-3