rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Basic Methods for the Biochemical Lab
  name:
    name part: Holtzhauer, Martin.
    role:
      role term: creator
    role:
      role term: author.
  name:
    name part: SpringerLink (Online service)
  type of resource: text
  origin info:
    place:
      place term: gw
    date issued: 2006
    edition: First English Edition.
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: electronic
    form: electronic resource
    reformatting quality: access
    form: remote
    extent: XVIII, 252 p. 23 illus., 1 illus. in color. online resource.
  abstract: Basic Methods for the Biochemical Lab elucidates proven lab procedures and practical hints for research in analytical and preparative biochemistry, as well as summarizing key data in numerous tables. To further emphasize the practical aspects and to minimize the text, theoretical introductions into the methods are mostly omitted. The chapters cover: quantitative methods (proteins, nucleic acids, phospholipids and carbohydrates), electrophoresis (several polyacrylamide and agarose systems, 2D-PAGE, detection methods and affinity electrophoresis), chromatographic protocols (thin-layer chromatography, GPC, IEC, affinity chromatography, HPLC), immunochemical protocols (hapten-carrier and enzyme conjugation, immunization and antibody purification, immune affinity chromatography, ELISA), centrifugation (differential and density gradient centrifugation for cells and cellular fractions), radioactivity (labeling and counting), buffers (buffer properties and compositions). In additional chapters, tables compile a broad array of useful information, e.g. SI units conversion factor, detergent, protein and nucleotide data, and the basic principles of statistics and enzyme and receptor kinetics are reviewed. This first English-language edition of a successful German-language manual will be a valuable resource for students and experts in biochemistry, biotechnology and biomedical laboratories.
  table of contents: Quantitative Methods -- Electrophoresis -- Chromatography -- Immunochemical Protocols -- Centrifugation -- Radioactive Labeling -- Buffers -- Tables -- Statistics and Data Analysis.
  note: by Martin Holtzhauer.
  subject:
    topic: Life sciences
  subject:
    topic: Laboratory medicine
  subject:
    topic: Analytical chemistry
  subject:
    topic: Biotechnology
  subject:
    topic: Medicinal chemistry
  subject:
    topic: Biochemistry
  subject:
    topic: Life Sciences
  subject:
    topic: Biochemistry, general
  subject:
    topic: Biotechnology
  subject:
    topic: Analytical Chemistry
  subject:
    topic: Medicinal Chemistry
  subject:
    topic: Laboratory Medicine
  classification: QH345
  classification: QD415-436
  classification: 572
  related item:
    title info:
      title: Springer eBooks
  related item:
    title info:
      title: Springer Lab Manuals
  identifier: 9783540327868
  identifier: http://dx.doi.org/10.1007/3-540-32786-X
  record info:
    record creation date: 100301
    record change date: 20151204181335.0
    record identifier: 978-3-540-32786-8