rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Gigaseal Formation in Patch Clamping
    sub title: With Applications of Nanotechnology
  name:
    name part: Malboubi, Majid.
    role:
      role term: creator
    role:
      role term: author.
  name:
    name part: Jiang, Kyle.
    role:
      role term: author.
  name:
    name part: SpringerLink (Online service)
  type of resource: text
  origin info:
    place:
      place term: gw
    date issued: 2014
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: electronic
    form: electronic resource
    reformatting quality: access
    form: remote
    extent: XI, 94 p. 81 illus., 65 illus. in color. online resource.
  abstract: This book presents an investigation of gigaseal formation using micro/nanotechnology. The aims of the book are twofold. First, it explains the mechanisms of gigaseal formation using the latest discoveries. Second, it provides practical techniques for frequent formation of high resistance seals. The formation of a high-resistance electrical seal, also known as a gigaseal, between a cell membrane and a glass micropipette tip is essential in patch-clamp experiments. Even though four decades have passed since the introduction of the patch-clamping technique by Neher and Sakmann, gigaseal formation remains an obstacle in developing the high-throughput ion channel screening systems required by the pharmaceutical industry. Here the authors share their latest methods for achieving gigaseal formation and describe techniques that are highly desirable at both research and industrial levels. Nanotechnology has been found to be a powerful tool for studying and modifying glass micropipettes and in tackling the problem of gigaseal formation.
  table of contents: Chapter 1 Introduction -- Chapter 2 Development of patch clamping -- Chapter 3 Gigaseal formation -- Chapter 4 Effect of roughness on gigaseal formation -- Chapter 5 Effect of hydrophilicIty on gigaseal formation -- Chapter 6 Effect of tip size on gigaseal formation -- Chapter 7 Study of glass micropipettes from tip formation to characterization -- References.
  note: by Majid Malboubi, Kyle Jiang.
  subject:
    topic: Engineering
  subject:
    topic: Nanotechnology
  subject:
    topic: Biomedical engineering
  subject:
    topic: Engineering
  subject:
    topic: Nanotechnology and Microengineering
  subject:
    topic: Biomedical Engineering
  classification: T174.7
  classification: 620.5
  related item:
    title info:
      title: Springer eBooks
  related item:
    title info:
      title: SpringerBriefs in Applied Sciences and Technology
  identifier: 9783642391286
  identifier: http://dx.doi.org/10.1007/978-3-642-39128-6
  record info:
    record creation date: 130724
    record change date: 20151103124530.0
    record identifier: 978-3-642-39128-6