rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Development of a Fully Integrated "Sample-In-Answer-Out" System for Automatic Genetic Analysis
  name:
    name part: Zhuang, Bin.
    role:
      role term: creator
    role:
      role term: author.
    role:
      role term: aut
    role:
      role term: http://id.loc.gov/vocabulary/relators/aut
  name:
    name part: SpringerLink (Online service)
  type of resource: text
  origin info:
    place:
      place term: si
    date issued: 2018
    edition: 1st ed. 2018.
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: electronic
    form: electronic resource
    reformatting quality: access
    form: remote
    extent: XXIII, 114 p. 58 illus., 44 illus. in color. online resource.
  abstract: This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an "in-situ" PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system's performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid "sample-in-answer-out" testing in routine clinical practice.
  table of contents: Introduction -- The construction of a general platform for capillary electrophoresis -- Integrated module for automatic DNA extraction and amplification -- A fully-integrated genetic analysis system -- Conclusion and prospects for future work.
  note: by Bin Zhuang.
  subject:
    topic: Biomedical engineering
  subject:
    topic: Genetic engineering
  subject:
    topic: Nucleic acids
  subject:
    topic: Biomedical Engineering and Bioengineering
  subject:
    topic: Genetic Engineering
  subject:
    topic: Nucleic Acid Chemistry
  classification: R856-857
  classification: HC79.E5
  classification: GE220
  classification: 610.28
  related item:
    title info:
      title: Springer eBooks
  related item:
    title info:
      title: Springer Theses, Recognizing Outstanding Ph.D. Research
  identifier: 9789811047534
  identifier: https://doi.org/10.1007/978-981-10-4753-4
  record info:
    record creation date: 170720
    record change date: 20191220125817.0
    record identifier: 978-981-10-4753-4