rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Enzyme-linked Immunosorbent Assay (ELISA)
    sub title: From A to Z
  name:
    name part: Hosseini, Samira.
    role:
      role term: creator
    role:
      role term: author.
    role:
      role term: aut
    role:
      role term: http://id.loc.gov/vocabulary/relators/aut
  name:
    name part: Vázquez-Villegas, Patricia.
    role:
      role term: author.
    role:
      role term: aut
    role:
      role term: http://id.loc.gov/vocabulary/relators/aut
  name:
    name part: Rito-Palomares, Marco.
    role:
      role term: author.
    role:
      role term: aut
    role:
      role term: http://id.loc.gov/vocabulary/relators/aut
  name:
    name part: Martinez-Chapa, Sergio O.
    role:
      role term: author.
    role:
      role term: aut
    role:
      role term: http://id.loc.gov/vocabulary/relators/aut
  name:
    name part: SpringerLink (Online service)
  type of resource: text
  origin info:
    place:
      place term: si
    date issued: 2018
    edition: 1st ed. 2018.
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: electronic
    form: electronic resource
    reformatting quality: access
    form: remote
    extent: XI, 115 p. 38 illus., 34 illus. in color. online resource.
  abstract: This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices. .
  table of contents: 1.Fundamentals of ELISA: from the evolution of the immunoassays until invention of ELISA -- 2.General overview on applications of ELISA -- 3.Step by step with ELISA: mechanism of operation, crucial elements, different protocols, and insights on immobilization and detection of various biomolecular entities -- 4. Evaluation of the detection results obtained from ELISA -- 5. Advantages, disadvantages and modifications of conventional ELISA -- 6.Common questions and answers about ELISA.
  note: by Samira Hosseini, Patricia Vázquez-Villegas, Marco Rito-Palomares, Sergio O. Martinez-Chapa.
  subject:
    topic: Biomedical engineering
  subject:
    topic: Immunology
  subject:
    topic: Biophysics
  subject:
    topic: Biological physics
  subject:
    topic: Proteins
  subject:
    topic: Biotechnology
  subject:
    topic: Bioinformatics
  subject:
    topic: Biomedical Engineering and Bioengineering
  subject:
    topic: Immunology
  subject:
    topic: Biological and Medical Physics, Biophysics
  subject:
    topic: Protein-Ligand Interactions
  subject:
    topic: Biotechnology
  subject:
    topic: Computational Biology/Bioinformatics
  classification: R856-857
  classification: HC79.E5
  classification: GE220
  classification: 610.28
  related item:
    title info:
      title: Springer eBooks
  related item:
    title info:
      title: SpringerBriefs in Forensic and Medical Bioinformatics
  identifier: 9789811067662
  identifier: https://doi.org/10.1007/978-981-10-6766-2
  record info:
    record creation date: 171230
    record change date: 20191021222128.0
    record identifier: 978-981-10-6766-2