rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Synthesis and Biological Evaluation of Pyrrole-Imidazole Polyamide Probes for Visualization of Telomeres
  name:
    name part: Kawamoto, Yusuke.
    role:
      role term: creator
    role:
      role term: author.
    role:
      role term: aut
    role:
      role term: http://id.loc.gov/vocabulary/relators/aut
  name:
    name part: SpringerLink (Online service)
  type of resource: text
  origin info:
    place:
      place term: si
    date issued: 2019
    edition: 1st ed. 2019.
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: electronic
    form: electronic resource
    reformatting quality: access
    form: remote
    extent: XII, 122 p. 62 illus., 52 illus. in color. online resource.
  abstract: This book presents a novel method of synthesizing sequence-specific DNA binding pyrrole-imidazole (Py-Im) polyamides and demonstrates polyamides targeting longer base pairs than those previously reported and their cellular application. It discusses the development of the facile synthetic method of tandem hairpin Py-Im polyamides and the method of visualizing telomeres under mild conditions without harsh denaturation, as well as research resulting in the synthesis not only of previously reported tandem dimer polyamides but also longer tandem trimer and tetramer polyamides. Using fluorescently labeled tandem hairpin Py-Im polyamides targeting a human telomere repetitive sequence, the author successfully stained telomeres in fixed cells without denaturation. These fluorescently labeled tandem tetramer polyamides are able to visualize human telomeres with lower background signals than previous polyamide probes, suggesting that they have higher specificity for telomeres. As such, this book provides insights into recent advance in Py-Im polyamides, particularly the extension of the target sites and improvement of the specificity, which can be applied to the study of pyrrole-imidazole polyamides with higher sequence specificity and the function of telomeres.
  table of contents: 1. General Introduction Sequence-specific DNA Binding Pyrrole-Imidazole Polyamides and Their Applications -- 2. Development of a New Method for Synthesis of Tandem Hairpin Pyrrole-Imidazole Polyamide Probes Targeting Human Telomeres -- 3. Tandem Trimer Pyrrole-Imidazole Polyamide Probes Targeting 18 Base Pairs in Human Telomere Sequences -- 4. Targeting 24 bp within Telomere Repeat Sequences with Tandem Tetramer Pyrrole−Imidazole Polyamide Probes. .
  note: by Yusuke Kawamoto.
  subject:
    topic: Bioorganic chemistry
  subject:
    topic: Nucleic acids
  subject:
    topic: Bioorganic Chemistry
  subject:
    topic: Nucleic Acid Chemistry
  classification: QD241-441
  classification: 547
  related item:
    title info:
      title: Springer eBooks
  related item:
    title info:
      title: Springer Theses, Recognizing Outstanding Ph.D. Research
  identifier: 9789811369124
  identifier: https://doi.org/10.1007/978-981-13-6912-4
  record info:
    record creation date: 190307
    record change date: 20190618131022.0
    record identifier: 978-981-13-6912-4