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Chromatin and chromatin remodeling enzymes
1 online resource (xxxviii, 540 pages, [32] pages of plates) : illustrations (some color).
DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the Chromatin remodellng enzymes play an important role in gene transcription. *Histone Bioinformatics *Biochemistry of histones, nucleosomes and chromatin *Molecular cytology of chromatin functions.
1. Mining core histone sequences from public protein databases -- 2. Reconstitution of nucleosome core particles from recombinant histones and DNA -- 3. Preparation and crystallization of nucleosome core particle -- 4. Creating designer histones by native chemical ligation -- 5. Two-dimensional gel analysis of histones and other H2AX-related methods -- 6. Assembly of yeast chromatin using ISWI complexes -- 7. Reconstitution of yeast chromatin using yNap1p -- 8. DNA synthesis-dependent and -independent chromatin assembly pathways in Xenopus egg extracts -- 9. Preparation of defined mononucleosomes, dinucleosomes, and nucleosome arrays in vitro and analysis of transcription factor binding -- 10. Purification of native, defined chromatin segments -- 11. Purification of defined chromosomal domains -- 12. Probing core histone tail-DNA interactions in a model dinucleosome system -- 13. Site-directed histone-DNA contact mapping for analysis of nucleosome dynamics -- 14. Site-specific attachment of reporter compounds to recombinant histones -- 15. Histone MacroH2A purification and nucleosome reconstitution -- 16. Analysis of histone variant H2A.Z localization and expression during early development -- 17. Histone variant CENP-A purification, nucleosome reconstitution -- 18. Immunological analysis and purification of centromere complex -- 19. Purification and analyses of histone H1 variants and H1 posttranslational modifications -- 20. HMGA proteins: isolation, biochemical modifications, and nucleosome interactions -- 21. Preparation and functional analysis of HMGN proteins -- 22. Methods for visualizing chromatin dynamics in living yeast -- 23. Direct visualization of transcription factor-induced chromatin remodeling and cofactor recruitment in vivo -- 24. Measuring histone and polymerase dynamics in living cells -- 25. Measurement of dynamic protein binding to chromatin in vivo, using photobleaching microscopy -- 26. Quantification of protein-protein and protein-DNA interactions in vivo, using fluorescence recovery after photobleaching -- 27. Fluorescence recovery after photobleaching (FRAP) methods for visualizing protein dynamics in living mammalian cell nuclei -- 28. Correlative light and electron spectroscopic imaging of chromatin in situ -- 29. RNA fluorscence in situ hybridization tagging and recovery of associated proteins to analyze in vivo chromatin interactions -- 30. 3C technology: analyzing the spatial organization of genomic loci in vivo / Erik Splinter, Frank Grosveld, and Wouter de Laat.
Includes bibliographical references and index.
Chromatin and chromatin remodeling enzymes. Part A
Amsterdam ; Boston : Elsevier Academic Press, ©2004