rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Manual of research techniques in cardiovascular medicine
  name:
    name part: Ardehali, Hossein.
    role:
      role term: edt
  name:
    name part: Bolli, Roberto.
    role:
      role term: edt
  name:
    name part: Losordo, Douglas.
    role:
      role term: edt
  type of resource: text
  genre: bibliography
  genre: Electronic books.
  origin info:
    place:
      place term: enk
    date issued: 2014
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource (473 pages) : illustrations
  abstract: While some research methods or techniques are applicable in several areas of medicine, research in cardiovascular diseases requires knowledge of an increasing array of procedures, techniques and measurements that are highly specialized and unique to this area of investigation. Edited by senior clinical investigators who are recognized leaders in cardiovascular medicine worldwide, this book provides readers with a comprehensive, practical "how-to-do-it" review of best-practice techniques for cardiovascular research.
  table of contents: Cover; Dedication; Title page; Copyright page; Contents; List of Contributors; Preface; About the Companion Website; Part 1: Electrophysiology; 1: Measurement of calcium transient ex vivo; Introduction; Preparation and fluorescent indicator loading; Optical setup; Calibration; Analysis and interpretation; Set-by-step procedure; References; 2: Confocal imaging of intracellular calcium cycling in isolated cardiac myocytes; Introduction; Materials and methods; Chamber, electrical stimulation, and superfusate delivery apparatus; Confocal microscope system.
  table of contents: Cell loading of fluorescent Ca2+ indicatorsExperimental approach; Data analyses; Weaknesses and strengths of the method and alternative approaches; Conclusions; References; 3: Generating a large animal model of persistent atrial fibrillation; Introduction; Insights from tachypacing-induced AF models; Protocol; Generating a tachypacing-induced AF model; Pacing protocols; Ventricular rate during AF: to ablate or not to ablate; Strengths and weaknesses of the model; Complications and alternative approaches to study AF; Potential complications; Alternative AF models; Conclusion; Acknowledgement.
  table of contents: Choices to make before an experimentAP recording procedure; Frequent problems; AP analysis; Alternative approaches and extensions; Weaknesses and strengths; Conclusion; References; 6: Patch-clamp recordings from isolated cardiac myocytes; Introduction; Theory of patch-clamp recording; Cell-attached configuration; Whole-cell recording configuration; Inside-out excised patch configuration; Outside-out excised patch configuration; Instrumentation for patch-clamp recordings; Experimental aspects of patch-clamp recordings; Gigaseal formation; Whole-cell recordings.
  table of contents: Whole-cell voltage-clamp protocols, data acquisition, and analysisConclusions and future directions; References; 7: Optical mapping of the heart; Introduction; Protocols; Experimental set-up; Tissue preparations (Figure 7.2); Staining and image acquisition; Data processing and analysis; Representative results; Alternative approaches; Weaknesses and strengths of method; Conclusions; References; Part 2: Isolation and Maintenance of Primary Stem Cells; 8: Isolation of colony-forming endothelial progenitor cells; Introduction; Materials; Laboratory reagents; Laboratory equipment; Methods.
  note: edited by Hossein Ardehali, Roberto Bolli, Douglas W. Losordo.
  note: Includes bibliographical references and index.
  subject:
    topic: Angioplasty
  subject:
    topic: Coronary heart disease
    topic: Treatment
  subject:
    topic: Tachycardia
    topic: Treatment
  subject:
    topic: Transluminal angioplasty
  subject:
    topic: Cardiovascular Diseases
  subject:
    topic: Cardiology
    topic: methods
  subject:
    topic: Models, Cardiovascular
  subject:
    topic: Research Design
  subject:
    topic: Medical
  subject:
    topic: MEDICAL
    topic: Surgery
    topic: General
  subject:
    topic: Cardiology
  subject:
    topic: Heart
    topic: Diseases
  classification: RD598.5 .M36 2014eb
  classification: 617.4 617.4/1059 617.41059
  classification: WG 120
  related item:
    title info:
      title: Manual of research techniques in cardiovascular medicine
    identifier: (DLC) 2013017936
  identifier: 9781118495162
  identifier: 1118495160
  identifier: 9781118495148
  identifier: 1118495144
  identifier: 9781118495131
  identifier: 1118495136
  identifier: 9781306219426
  identifier: 1306219426
  identifier: 9780470672693
  identifier: 0470672692
  identifier: EB00211204 Recorded Books
  identifier: https://doi.org/10.1002/9781118495148
  record info:
    record content source: E7B
    record creation date: 131219
    record change date: 20170124070701.1
    record identifier: ocn868925744
    language of cataloging:
      language term: eng