http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
Progress in Molecular Biology and Translational Science provides a forum for discussion of new discoveries, approaches, and ideas in molecular biology. It contains contributions from leaders in their fields and abundant references. Volume 126 features in-depth reviews that focus on the tools required to investigate mechanotransduction. Additional chapters focus on how we can use these tools to answer fundamental questions about the interaction of physical forces with cell biology, morphogenesis, and function of mature structures. Chapters in the volume are authored by a unique.
Front Cover; Mechanotransduction; Copyright; Contents; Contributors; Preface; Part One: Subcellular Tools for Activating and Measuring Mechanotransductive Signaling; Chapter One: The Detection and Role of Molecular Tension in Focal Adhesion Dynamics; 1. Brief Introduction to Mechanobiology; 2. Focal Adhesions in Mechanosensing; 2.1. Focal adhesion structure; 2.2. Molecular mechanisms of mechanotransduction; 3. Design and Use of Optically Based Molecular Tension Sensors; 3.1. Basics of Forster Resonance Energy Transfer (FRET); 3.2. Designs of FRET-based force-sensitive biosensors.
3.2.1. Extensible domain3.2.2. Rotatable domain; 3.2.3. Other designs; 3.3. Use of FRET-based tension sensors: Relative versus absolute measurements; 3.4. Critical control experiments and assumptions involved in the creation and use of FRET-based biosensors; 3.5. Conformation sensors versus tension sensors; 4. The Role of Molecular Tension in Focal Adhesion Dynamics; 5. Future Outlook; Acknowledgments; References; Chapter Two: Single-Cell Imaging of Mechanotransduction in Endothelial Cells; 1. Introduction; 2. Atherosclerosis, EC Wound Healing, and Mechanotransduction.
3. Signaling Molecules Involved in Mechanosensing and Mechanotransduction4. The Effect of Subcellular Structure on Mechanotransduction; 5. Focal Adhesion and FAK; 6. Tools to Monitor Signal Transduction in Live Cells; 6.1. FPs, FRET, and fluorescence lifetime imaging microscopy; 6.2. Quantitative image-based analysis for live cells; 6.3. The FRAP analysis and finite-element-based diffusion analysis; 6.4. Automatic tracking of moving cells and subcellular features; 7. Conclusion; References; Part Two: Focal Adhesions as Sensors; Chapter Three: Focal Adhesions Function as a Mechanosensor.
1. Introduction: The Basic Organization of Focal Adhesions2. Mechanosensitivity of Focal Adhesions; 3. Focal Adhesions and the Effects of Environmental Parameters; 4. Focal Adhesion Signals and Cell Migration; Acknowledgments; References; Chapter Four: Mechanosensation: A Basic Cellular Process; 1. Introduction; 1.1. Historical development; 1.2. Mechanosensation/-transduction; 1.3. Effects of extracellular matrix stiffness; 1.4. Stress generated by external compression/contractility; 1.5. Stress generated by cell contractility; 1.6. Biological relevance of external and internal stress.
2. Focal Adhesions2.1. Mechanotransduction/-signaling; 2.2. Focal adhesion proteins; 2.2.1. Vinculin; 2.2.2. Zyxin; 2.2.3. Talin; 2.2.4. Paxillin, Pyk2; 2.2.5. p130Cas; 2.2.6. Focal adhesion kinase; 2.3. Force transduction at focal adhesions; 2.4. Protein crosstalk; 2.5. Cell signaling pathways; 2.6. Translation of information gathered at focal adhesions; 2.7. Focal adherence junctions; 2.8. Measuring mechanotransduction/-sensation; 2.8.1. Flow chambers and cone and plate rheometers; 2.8.2. Magnetic and optical traps; 2.8.3. Atomic force microscopy and biomembrane force probe.
Includes bibliographical references at the end of each chapters and index.
Progress in molecular biology and translational science ; volume 126