rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    non sort: The
    title: use of CRISPR/cas9, ZFNs, TALENs in generating site specific genome alterations
  name:
    name part: Doudna, Jennifer A.
    role:
      role term: editor.
  name:
    name part: Sontheimer, Erik J.
    role:
      role term: editor.
  name:
    name part: Anders, Carolin
    role:
      role term: contributor.
  type of resource: text
  genre: bibliography
  genre: Electronic books.
  genre: Laboratory manuals.
  origin info:
    place:
      place term: ne
    date issued: 2014
    copyright date: 2014
    edition: First edition.
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource (594 pages, 24 unnumbered pages of plates) : illustrations (some color).
  abstract: This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells.
  table of contents: In vitro enzymology of Cas9 -- Targeted genome editing in human cells using CRISPR/Cas nucleases and truncated guide RNAs -- Determining the specificities of TALENs, Cas9, and other genome-editing enzymes -- Genome engineering with custom recombinases -- Genome engineering in human cells -- Genome editing in human stem cells -- Tagging endogenous loci for live-cell fluorescence imaging and molecule counting using ZFNs, TALENs, and Cas9 -- Genome editing using Cas9 nickases -- Assaying break and nick-induced homologous recombination in mammalian cells using the DR-GFP reporter and Cas9 nucleases -- Adapting CRISPR/Cas9 for functional genomics screens -- The iCRISPR platform for rapid genome editing in human pluripotent stem cells -- Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired Nicks -- Genome editing for human gene therapy -- Generation of site-specific mutations in the rate genome via CRISPR/Cas9 -- CRISPR/Cas9-based genome editing in mice by single plasmid injection -- Imaging genomic elements in living cells using CRISPR/Cas9 -- Cas9-based genome editing in xenopus tropicalis -- Cas9-based genome editing in zebrafish -- Cas9-based genome editing in drosophila -- Transgene-free genome editing by germline injection of CRISPR/Cas RNA -- Cas9-based genome editing in arabidopsis and tobacco -- Multiplex engineering of industrial yeast genomes using CRISPRm -- Protein engineering of Cas9 for enhanced function.
  note: edited by Jennifer A. Doudna, Erik J. Sontheimer ; contributors, Carolin Anders [and seventy-six others].
  note: Includes bibliographical references at the end of each chapter and indexes.
  subject:
    topic: Genetic engineering
    genre: Laboratory manuals
  subject:
    topic: Genomics
    genre: Laboratory manuals
  subject:
    topic: Endonucleases
    genre: Laboratory manuals
  subject:
    topic: Restriction enzymes, DNA
    genre: Laboratory manuals
  subject:
    topic: Genomics
  subject:
    topic: SCIENCE
    topic: Chemistry
    topic: Industrial & Technical
  subject:
    topic: TECHNOLOGY & ENGINEERING
    topic: Chemical & Biochemical
  subject:
    topic: Endonucleases
  subject:
    topic: Genetic engineering
  subject:
    topic: Genomics
  subject:
    topic: Restriction enzymes, DNA
  subject:
    topic: Genetic Engineering
  subject:
    topic: CRISPR-Cas Systems
  subject:
    topic: Clustered Regularly Interspaced Short Palindromic Repeats
  subject:
    topic: DNA Restriction Enzymes
  subject:
    topic: Endonucleases
  subject:
    topic: Genomics
  classification: QH442 .U84 2014eb
  classification: 572.86
  classification: QU 25
  related item:
    title info:
      title: Use of CRISPR/cas9, ZFNs, TALENs in generating site specific genome alterations
    origin info:
      publisher: San Diego, California : Academic Press, ©2014
      edition: First edition.
    physical description:
      extent: xx, 549 pages
    identifier: 0076-6879
    identifier: (OCoLC)885225075
  related item:
    title info:
      title: Methods in enzymology ; v. 546
  identifier: 9780128013342
  identifier: 0128013346
  identifier: 9780128011850
  identifier: https://www.sciencedirect.com/science/bookseries/00766879/546
  record info:
    record content source: E7B
    record creation date: 141203
    record change date: 20180501122022.0
    record identifier: ocn899004371
    language of cataloging:
      language term: eng