rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: G protein-coupled receptors
    sub title: signaling, trafficking and regulation
  name:
    name part: Shukla, Arun K.
    role:
      role term: editor.
  type of resource: text
  genre: bibliography
  genre: Electronic books.
  origin info:
    place:
      place term: mau
    date issued: 2016
    edition: First edition.
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource (480 pages) : color illustrations.
  table of contents: Front Cover; Series Editors; Methods in Cell Biology G Protein-Coupled Receptors: Signaling, Trafficking and Regulation; Copyright; Contents; Contributors; Preface; 01 -- Trafficking, Localization andImaging of GPCRs; 1 -- Localization and signaling of GPCRs in lipid rafts; INTRODUCTION; 1. LOCALIZATION OF GPCRS IN LIPID RAFTS; 1.1 Isolation of Lipid Rafts; 1.1.1 Detergent-free method; 1.1.2 Detergent-based method; 1.1.3 Immunoblotting and data interpretation; 1.2 Localization of GPCRs in Lipid Rafts; 1.2.1 Cells in suspension; 1.2.2 Adherent cells; 2. GPCR SIGNALING IN LIPID RAFTS
  table of contents: 2.1 Perturbation of Raft Stability2.2 Changing the Cholesterol Content; 2.3 Fluorescence Imaging; REFERENCES; 2 -- Imaging GPCRs trafficking and signaling with total internal reflection fluorescence microscopy in cultured neurons; 1. IMAGE ACQUISITION; 1.1 Important Considerations before Imaging; 1.2 Materials; 1.2.1 Cell culture; 1.2.2 TIRF microscopy equipment and settings; 1.3 Imaging; 1.3.1 Cell culture; 1.3.2 Live cell imaging; 1.4 Notes; 2. IMAGE ANALYSIS; 3. FINAL COMMENTS; Acknowledgments; REFERENCES; 3 -- Trafficking of ciliary G protein-coupled receptors; INTRODUCTION
  table of contents: 1. RHODOPSIN AS A CILIARY GPCR2. CILIARY GPCRS IN OTHER NEURONS; 3. OLFACTORY SENSORY NEURON CILIARY GPCRS; 4. DYNAMIC TRAFFICKING OF CILIARY GPCRS; CONCLUSIONS AND FUTURE DIRECTIONS; REFERENCES; 4 -- Single-molecule resolution of G protein-coupled receptor (GPCR) complexes; INTRODUCTION; 1. IMAGING METHODOLOGIES TO STUDY SINGLE GPCR MOLECULES; 1.1 Current Approaches to Visualize Individual GPCRs as Monomers, Dimers, and Oligomers; 1.2 Super-Resolution Imaging of Single Molecules: Resolving Lots and Lots of Spots...; 2. METHODS FOR PHOTOACTIVATED DYE-LOCALIZATION MICROSCOPY
  table of contents: 2.1 Seeing Single Molecules2.1.1 Choice of probe and labeling; 2.1.2 Optimization steps-labeling cells, blocking buffers, and fixatives; 2.1.3 Imaging considerations and conditions; 2.2 Counting Single Molecules; 2.2.1 Resolving single-molecule data; 2.2.2 Custom quantification of GPCR monomers, dimers, and oligomers; CONCLUSIONS AND PERSPECTIVES; Acknowledgments; REFERENCES; 5 -- Quantification of the mRNA expression of G protein-coupled receptors in human adipose tissue; INTRODUCTION; 1. MATERIALS; 1.1 Handling of Adipose Tissue Biopsies; 1.2 Adipose Tissue Homogenization
  table of contents: 1.3 Adipose Tissue RNA Purification and cDNA Generation1.4 qPCR Quantification of GPCR Expression Relative the Adipose Tissue Housekeeping Gene PPIA; 2. METHODS; 2.1 Handling of Adipose Tissue Biopsies; 2.2 Adipose Tissue Homogenization; 2.3 Adipose Tissue RNA Purification and cDNA Generation; 2.4 qPCR Quantification of GPCR Expression Relative to the Housekeeping Gene PPIA; 2.4.1 Screening of adipose tissue GPCR mRNA expression using pooled cDNA; 2.4.2 Quantification of GPCR expression relative to the expression of the adipose tissue housekeeping gene PPIA
  note: edited by Arun K. Shukla.
  note: Includes bibliographical references and index.
  subject:
    topic: G proteins
    topic: Receptors
  subject:
    topic: Cytology
    topic: Technique
  subject:
    topic: Cell receptors
  subject:
    topic: SCIENCE / Life Sciences / Anatomy & Physiology
  subject:
    topic: G proteins
    topic: Receptors
  subject:
    topic: Cell receptors
  subject:
    topic: Cytology
    topic: Technique
  subject:
    topic: GTP-Binding Proteins
  subject:
    topic: Cytological Techniques
  subject:
    topic: Receptors, Cell Surface
  classification: QP552.G16 G22 2016
  classification: QH585 .M47 v.132
  classification: 572/.696
  classification: 571.6
  related item:
    title info:
      title: G Protein-Coupled Receptors: Signaling, Trafficking and Regulation
    name:
      name part: Shukla, Arun K.
    origin info:
      publisher: : Elsevier Science,c2016
  related item:
    title info:
      title: Methods in cell biology ; v. 132
  identifier: 9780128036129
  identifier: 0128036125
  identifier: 9780128035955
  identifier: https://www.sciencedirect.com/science/bookseries/0091679X/132
  record info:
    record content source: N$T
    record creation date: 160302
    record change date: 20180501122029.0
    record identifier: ocn942588196
    language of cataloging:
      language term: eng