http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
Cell Polarity and Morphogenesis, the latest volume in the Methods in Cell Biology series, looks at cell polarity and morphogenesis. Edited by leaders in the field, this volume provides proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies.
Front Cover; Methods in Cell Biology; Series Editors; Methods in Cell BiologyCell Polarity and MorphogenesisVolume 139Edited byThomas LecuitIBDM (Institut de Biologie du Dévelop ...; Contents; Contributors; 1 -- A user's guide for characterizing plasma membrane subdomains in living cells by spot variation fluorescence cor ...; INTRODUCTION; 1. BUILDING A SENSITIVE AND ROBUST HOMEMADE SPOT VARIATION FLUORESCENCE CORRELATION SPECTROSCOPY/FLUORESCENCE RECOVERY AFTER P ...; 1.1 Description of the Optical Bench and Signal Collection; 1.2 Common Pitfalls and Practical Considerations
2. SAMPLE PREPARATIONS AND LABELING2.1 General Considerations on Sample Preparations; 2.2 Use of Fluorescent Proteins; 2.3 Membrane Labeling With Lipid Probes. Impact on Fluorescence Correlation Spectroscopy Measurements; 2.4 Surface Labeling of Nonadherent Cells With Antibodies Prior to Fluorescence Correlation Spectroscopy Measurements; 2.5 Pharmacological Treatments; 2.5.1 Latrunculin A treatment (actin meshwork disassembly); 2.5.2 Sphingolipid and cholesterol metabolism inhibition; 3. FLUORESCENCE CORRELATION SPECTROSCOPY RECORDING; 3.1 Preliminary Recording; 3.2 Spot Size Calibration
3.3 Fluorescence Correlation Spectroscopy Acquisitions at the Plasma Membrane of Living Cells3.4 Spot Fluorescence Recovery After Photobleaching Measurements at the Plasma Membrane of Living Cells; 4. DATA ANALYSIS AND INTERPRETATION; 4.1 Data Analysis and Fitting Procedures; 4.2 Data Interpretation; 4.3 Comparison of Spot Variation Fluorescence Correlation Spectroscopy Results; CONCLUSION; Acknowledgments; REFERENCES; 2 -- Investigating symmetry breaking in yeast: from seeing to understanding; INTRODUCTION; 1. GENETIC TOOLS; 2. MICROSCOPY-BASED METHODS TO VISUALIZE CELL POLARIZATION
2.1 Single Cell Time-Lapse Imaging2.1.1 Slide preparation for short-time lapse imaging; 2.1.2 Slide preparation for long time-lapse imaging; 2.2 Bulk Cell Polarization Assays; 2.3 Fluorescence Recovery After Photobleaching; 2.3.1 Practical considerations for fluorescence recovery after photobleaching in Saccharomyces cerevisiae; 2.3.2 Mathematical modeling of the fluorescence recovery after photobleaching data; 2.4 Fluorescence Correlation Spectroscopy; 2.4.1 Requirements for fluorescence correlation spectroscopy; 2.4.2 Fluorescence cross-correlation spectroscopy
2.4.3 Requirements and analysis of fluorescence cross-correlation spectroscopy2.5 Fluorescence Resonance Energy Transfer; 2.5.1 Design of fluorescence resonance energy transfer biosensor for the guanine nucleotide exchange factor activity toward Cdc42; 2.5.2 Fluorescence resonance energy transfer experiments with the Cdc42 activation biosensor; CONCLUSION AND FUTURE PERSPECTIVE; NOTES; Acknowledgments; REFERENCES; 3. Precise tracking of the dynamics of multiple proteins in endocytic events; INTRODUCTION; 1. RATIONALE; 2. SAMPLE PREPARATION; 3. FLUORESCENCE MICROSCOPY; 3.1 Microscopy Setup
Includes bibliographical references and index.
SCIENCE / Life Sciences / Anatomy & Physiology