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Chemical and biochemical approaches for the study of anesthetic function
Intro; Title page; Table of Contents; Copyright; Contributors; Preface; Section I: Structural Approaches; Chapter One: Structural Analysis of Anesthetics in Complex with Soluble Proteins; Abstract; 1 Introduction; 2 Crystallographic Issues Relevant to Analysis of Anesthetic Binding; 3 Crystallizing Apoferritin with Isoflurane and Propofol; 4 Protocol; 5 Analysis of the Structure; 6 Summary; Acknowledgment; Chapter Two: X-Ray Crystallographic Studies for Revealing Binding Sites of General Anesthetics in Pentameric Ligand-Gated Ion Channels; Abstract; 1 Introduction.
2 Production of Monodisperse pLGICs with High Purity3 Crystallization of pLGICs in the Absence and Presence of General Anesthetics; 4 Functional Validation of the Bound Anesthetics; 5 Case Studies; Acknowledgments; Chapter Three: Solution NMR Studies of Anesthetic Interactions with Ion Channels; Abstract; 1 Introduction; 2 Preparation of Ion Channels for Solution NMR; 3 NMR Methods to Identify Anesthetics Binding Sites and Detect Anesthetic-Induced Dynamics Changes; 4 Case Studies; Acknowledgments.
Chapter Four: Time-Resolved Neutron Interferometry and the Mechanism of Electromechanical Coupling in Voltage-Gated Ion ChannelsAbstract; 1 Introduction; 2 Methods; 3 Results; 4 Discussion; 5 Conclusions and Future Prospects; Acknowledgments; Section II: Evolving Biophysical Technologies; Chapter Five: Fluorescent Anesthetics; Abstract; 1 Introduction; 2 Value of 1-AMA for Studying General Anesthetic Mechanisms; 3 Value of 1-AZA for Studying General Anesthetic Mechanisms; 4 Experiments; Chapter Six: Investigation of Anesthetic-Protein Interactions by a Thermodynamic Approach; Abstract.
1 Introduction2 ITC Measurements; 3 Conclusion; Chapter Seven: Electron Spin Resonance (EPR) in Drosophila and General Anesthesia; Abstract; 1 Introduction; 2 Electron Spin Resonance Primer; 3 Quantitation; 4 Sample Preparation; 5 Measurements; 6 Origin of the Spin Signal in Drosophila; 7 Conclusions; Chapter Eight: Giant Plasma Membrane Vesicles: An Experimental Tool for Probing the Effects of Drugs and Other Conditions on Membrane Domain Stability; Abstract; 1 Introduction; 2 GPMV Formation; 3 Measuring GPMV Miscibility Transition Temperatures; 4 Summary and Conclusion; Acknowledgments.
Section III: In vivo Technologies Established and Evolving; Chapter Nine: Anesthetic Drug Discovery and Development: A Case Study of Novel Etomidate Analogs; Abstract; 1 Introduction; 2 Etomidate Analog Design Strategies; 3 Testing Novel Etomidate Analogs for Hypnotic Activity Using Loss of Righting Reflexes Assays; 4 Testing Novel Etomidate Analogs for Adrenocortical Inhibitory Activity; 5 Summary and Conclusion; Acknowledgments; Chapter Ten: Optoanesthesia: Use of Anesthetic Photolabels In Vivo; Abstract; 1 Introduction; 2 Anesthetic Photolabel Availability.
edited by Roderic G. Eckenhoff, Ivan J. Dmochowski.
Chemical and biochemical approaches for the study of anesthetic function. Part B
Cambridge, MA : Academic Press, an imprint of Elsevier, 2018