Cell growth, differentiation and senescence : a practical approach /

• Άλλοι συγγραφείς: Studzinski, George P. (επιμελητής.)
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Oxford ; New York : Oxford University Press, 1999.
• Σειρά: Practical approach series 215.
• Θέματα: Κυτταρική διαφοροποίηση; Κυτταρικός θάνατος; Ηλεκτρονικά βιβλία; Εργαστηριακά εγχειρίδια

 There are three main themes running through this volume. First, basic methods for measurement of cell proliferation are introduced and explained with reference to various systems, primarily in vitro, but in vivo procedures are also illustrated. The second theme is growth signalling, and is exemplified by methods for the analysis of transduction pathways for growth, beginning at the cell membrane and leading to the cell nucleus. The last theme presented here is growth cessation, illustrated by several systems for induction of cell differentiation, and of cell senescence. The emphasis throughout the book is on human cell systems, making it particularly relevant to scientists interested in human disease, especially cancer. Importantly, well proved methods for studying cell growth are supplemented by some novel approaches, e.g., studies of cell cycle checkpoints, cell spheroids, and nuclear architecture. Only two chapters have been retained, in an updated form from Cell Growth and Apoptosis, the predecessor volume.; The book is written by a team of scientists highly experienced in procedures they describe, and offer details and hints found valuable in their own laboratories; thus, variants of the same general methods can be found in different chapters. These should be helpful to beginning as well as experienced investigators, and are designed to stimulate new approaches to old and new questions. 

 Selection of methods for measuring proliferation / Robert Wieder -- Cell growth and cytotoxicity assays / Philip Skehan -- Cell growth and kinetics in multicell spheroids / Ralph E. Durand -- Assessment of DNA damage cell cycle checkpoints in G1 and G2 phases of mammalian cells / Patrick M. O'Connor and Joany Jackman -- Assessment of the role of growth factors and their receptors in cell proliferation / Subal Bishayee -- Analysis of growth-signalling protein kinase cascades / Alessandro Alessandrini -- Cytometric analysis of the pRB pathway / Gloria Juan, Frank Traganos, and Zbigniew Darzynkiewicz -- Methods for oncogene detection / Stephen C. Cosenza, Stacey J. Baker, and E. Premkumar Reddy -- Isolation and visualization of the principal components of nuclear architecture / Gary S. Stein [and others] -- Antisense oligonucleotides : tools for elucidating gene function / John J. Wolf and W. Michael Flanagan -- Human leukaemia cells as a model differentiation system / Dorothy C. Moore and George P. Studzinski -- Induction of differentiation of human intestinal cells in vitro / Heide S. Cross and Enik�o Kallay -- Proliferation and differentiation of human prostate cells / Therese Thalhammer and Heide S. Cross -- Senescence and immortalization of human cells / Karen Hubbard and Harvey L. Ozer.