Protein localization by fluorescence microscopy : a practical approach /

• Άλλοι συγγραφείς: Allan, Victoria J.
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Oxford ; New York : Oxford University Press, �2000.
• Σειρά: Practical approach series ; 218.
• Θέματα: Proteins > Analysis.; Fluorescence microscopy.; Green fluorescent protein.; Immunofluorescence.; SCIENCE > Life Sciences > Biochemistry.; Sequence Analysis, Protein.; Microscopy, Fluorescence.; Luminescent Proteins.; Electronic books.

There is an ever-increasing number of genes that have been sequenced but are of completely unknown function. The ability to determine the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is a vital step t...

Πίνακας περιεχομένων:

• Machine generated contents note: 1. Basic immunofluorescence / Victoria J. Allan
• 2. Immunofluorescent labelling of sections / J. Victor Small / Alison J. North
• 3. Simultaneous in situ detection of DNA and proteins / Klaus Ersfeld / Elisa M. Stone
• 4. Instruments for fluorescence imaging / W. B. Amos
• 5. Fluorescence microscopy of living vertebrate cells / David Shima / Rainer Pepperkok
• 6. Visualizing fluorescence in Drosophila
• optimal detection in thick specimens / Ilan Davis
• 7. Green fluorescent protein in plants / Chris Hawes / Petra Boevink / Ian Moore
• 8. Fluorescence microscopy in yeast / Iain M. Hagan / Kathryn R. Ayscough
• App. A1 Excitation and emission maxima of commonly used fluorophores
• App. A2 Commercial sources of antibodies
• App. A3 Optical units
• App. A4 List of Suppliers.