Protein phosphorylation : a practical approach /

• Άλλοι συγγραφείς: Hardie, D. G. (D. Grahame)
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: London ; New York : Oxford University Press, 1999.
• Έκδοση: 2nd ed.
• Σειρά: Practical approach series ; 211.
• Θέματα: Phosphoproteins > Laboratory manuals.; Phosphorylation > Laboratory manuals.; Protein kinases > Laboratory manuals.; Phosphoprotein phosphatases > Laboratory manuals.; SCIENCE > Life Sciences > Biochemistry.; Phosphoprotein phosphatases.; Phosphoproteins.; Phosphorylation.; Protein kinases.; Fosforylering.; Eiwitten.; Prote�ine-fosfatasen.; Eiwitkinasen.; Prot�eines.; Phosphoprot�eine phosphatase.; Prot�eine kinase.; Phosphoprot�eines > Manuels de laboratoire.; Prot�eines kinase > manuels de laboratoire.; Methode; Phosphorylierung; Proteine; Proteinkinasen; Protein Kinases.; Electronic books.; Laboratory manuals.

 Study of protein phosphorylation in intact cells -- Analysis of signal transduction pathways using protein kinase inhibitors and activators -- Analysis of signal transduction pathways using protein-serin/threonine phosphatase inhibitors -- Analysis of signal transduction pathways using molecular biological manipulations -- Phosphopeptide mapping and phosphoamino acid analysis on cellulose thin-layer plates -- Phosphorylation site analysis by mass spectrometry -- Assay and purification of protein (serine/threonine) phosphatases -- Identification of substrates for protein-tyrosine phosphateases -- Assay and purification of protein-serine/threonine kinases -- Assay of protein kinases and phosphatases using specific peptide substrates -- Cloning and expression of cDNAs encoding protein kinase subunits -- Interaction cloning of protein kinase partners -- Identifying protein kinase substrates by expression screening with solid-phase phosphorylation -- Analysis of protein kinase interactions using the two-hybrid method -- Analysis of protein kinase interactions using biomolecular interaction analysis -- Analysis of protein kinase specificity using oriented peptide libraries -- Crystallization of protein kinases and phosphatases.