Protein phosphorylation : a practical approach /

• Άλλοι συγγραφείς: Hardie, D. G. (D. Grahame)
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: London ; New York : Oxford University Press, 1999.
• Έκδοση: 2nd ed.
• Σειρά: Practical approach series ; 211.
• Θέματα: Phosphoproteins > Laboratory manuals.; Phosphorylation > Laboratory manuals.; Protein kinases > Laboratory manuals.; Phosphoprotein phosphatases > Laboratory manuals.; SCIENCE > Life Sciences > Biochemistry.; Phosphoprotein phosphatases.; Phosphoproteins.; Phosphorylation.; Protein kinases.; Fosforylering.; Eiwitten.; Prote�ine-fosfatasen.; Eiwitkinasen.; Prot�eines.; Phosphoprot�eine phosphatase.; Prot�eine kinase.; Phosphoprot�eines > Manuels de laboratoire.; Prot�eines kinase > manuels de laboratoire.; Methode; Phosphorylierung; Proteine; Proteinkinasen; Protein Kinases.; Electronic books.; Laboratory manuals.

Πίνακας περιεχομένων:

• Study of protein phosphorylation in intact cells
• Analysis of signal transduction pathways using protein kinase inhibitors and activators
• Analysis of signal transduction pathways using protein-serin/threonine phosphatase inhibitors
• Analysis of signal transduction pathways using molecular biological manipulations
• Phosphopeptide mapping and phosphoamino acid analysis on cellulose thin-layer plates
• Phosphorylation site analysis by mass spectrometry
• Assay and purification of protein (serine/threonine) phosphatases
• Identification of substrates for protein-tyrosine phosphateases
• Assay and purification of protein-serine/threonine kinases
• Assay of protein kinases and phosphatases using specific peptide substrates
• Cloning and expression of cDNAs encoding protein kinase subunits
• Interaction cloning of protein kinase partners
• Identifying protein kinase substrates by expression screening with solid-phase phosphorylation
• Analysis of protein kinase interactions using the two-hybrid method
• Analysis of protein kinase interactions using biomolecular interaction analysis
• Analysis of protein kinase specificity using oriented peptide libraries
• Crystallization of protein kinases and phosphatases.