Green fluorescent protein properties, applications, and protocols

• Corporate Author: Wiley InterScience (Online service)
• Other Authors: Chalfie, Martin, Kain, Steven
• Format: Electronic Book
• Language: English
• Published: Hoboken, N.J. Wiley-Interscience c2006
• Edition: 2nd ed
• Series: Methods of biochemical analysis v. 47
• Subjects: Βιοχημεία; Πρωτεΐνες > Ανάλυση, Χημική; Πρωτεΐνες > Βιοσύνθεση

Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species

Table of Contents:

• Discovery of green fluorescent protein / Osamu Shimomura
• Photons for reporting molecular events : green fluorescent protein and four luciferase systems / J. Woodland Hastings and James G. Morin
• Biochemical and physical properties of green fluorescent protein / William W. Ward
• The three-dimensional structure of green fluorescent protein and its implications for function and design / George N. Phillips, Jr.
• Molecular biology and mutation of green fluorescent protein / David A. Zacharias, Roger Y. Tsien
• Discovery and properties of GFP-like proteins from nonbioluminescent anthozoa / Konstantin A. Lukyanov ... [et al.]
• Evolution of function and color in GFP-like proteins / Mikhail V. Matz, Yulii A. Labas, and Juan Ugalde
• The uses of green fluorescent protein in prokaryotes / Raphael H. Valdivia, Brendan P. Cormack, and Stanley Falkow
• The uses of green fluorescent protein in yeasts / Amy L. Hitchcock, Jason A. Kahana, and Pamela A. Silver
• Uses of GFP in Caenorhabditis elegans / Oliver Hobert and Paula Loria
• Green fluorescent protein applications in Drosophila / Tulle Hazelrigg and Jennifer H. Mansfield
• The uses of green fluorescent protein in plants / Jim Haseloff and Kirby R. Siemering
• Uses of GFP in transgenic vertebrates / Sean Magason ... [et al.]
• The uses of green fluorescent protein in mammalian cells / Theresa H. Ward and Jennifer Lippincott-Schwartz
• Practical considerations for use of reef coral fluorescent proteins in mammalian cells : applications in fluorescent microscopy and flow cytometry / Yu Fang ... [et al.]
• Pharmaceutical applications of GFP and RCFP / Nicola Bevan and Stephen Rees
• Reassembled GFP : detecting protein-protein interactions and protein expression patterns / Thomas J. Magliery and Lynne Regan