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03261nam a22005535i 4500 |
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978-0-306-47595-5 |
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DE-He213 |
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20151204173036.0 |
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cr nn 008mamaa |
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100301s2001 ne | s |||| 0|eng d |
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|a 9780306475955
|9 978-0-306-47595-5
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|a 10.1007/0-306-47595-2
|2 doi
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|a 543.65
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|a Mass Spectrometry and Genomic Analysis
|h [electronic resource] /
|c edited by J. Nicholas Housby.
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|a Dordrecht :
|b Springer Netherlands,
|c 2001.
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|a XV, 149 p.
|b online resource.
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|a text
|b txt
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|a Focus on Structural Biology,
|x 1571-4853 ;
|v 2
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|a 76 2. Short Oligonucleotide Mass Analysis 76 2. 1. Method Outline 76 2. 2. Design of PCR Primers and Fragments for Analysis 78 2. 3. Typical PCR Reaction Conditions 79 3. Electrospray Ionisation Mass Spectrometry 79 Formation of Ions 3. 1. 79 3. 2. Tandem Mass Spectrometry 79 3. 3. Typical ESI-MS Settings for SOMA 80 4. Purification Procedures 80 4. 1. Phenol/Chloroform Extraction and Ethanol Precipitation 80 4. 2. In-line HPLC Purification 81 5. Genotyping Using SOMA 81 5. 1. APC Genotyping in Human Subjects 81 5. 2. APC Genotyping in Min Mice 85 5. Mutation Detection Using SOMA 86 6. 1. Analysis of p53 Mutations in Liver Cancer Patients 86 6. 1. 1. p53 Mutations in Liver Tumours 87 6. 1. 2. p53 Mutations in Plasma Samples 88 7. Advantages and Disadvantages of SOMA 89 8. Future Perspectives 90 9. Acknowledgements 91 10. References 91 CHAPTER 7 WV. Bienvenut, M. Müller, PM. Palagi, E. Gasteiger, M. Heller, E. Jung, M. Giron, R. Gras, S. Gay, PA. Binz, G J. Hughes, JC. Sanchez, RD. Appel, DF. Hochstrasser Proteomics and Mass Spectrometry: Some Aspects and Recent Developments 1. Introduction to Proteomics 93 2. Protein Biochemical and Chemical Processing Followed by Mass Spectrometric Analysis 94 2. 1. 2-DE Gel Protein Separation 95 Protein Identification Using Peptide Mass Fingerprinting and Robots 96 2. 2. 2. 2. 1. MALDI-MS Analysis 98 2. 2. 2. MS/MS Analysis 102 Improvement of the Identification by Chemical Modification of Peptides 106 2. 2. 3.
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|a Chemistry.
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| 650 |
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|a Human genetics.
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| 650 |
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|a Analytical chemistry.
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|a Mass spectrometry.
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|a Life sciences.
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|a Biochemistry.
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|a Animal anatomy.
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|a Chemistry.
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| 650 |
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|a Mass Spectrometry.
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|a Life Sciences, general.
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| 650 |
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|a Analytical Chemistry.
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| 650 |
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|a Human Genetics.
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| 650 |
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|a Animal Anatomy / Morphology / Histology.
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| 650 |
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|a Biochemistry, general.
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| 700 |
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|a Nicholas Housby, J.
|e editor.
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| 710 |
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|a SpringerLink (Online service)
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|t Springer eBooks
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|i Printed edition:
|z 9780792371731
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| 830 |
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|a Focus on Structural Biology,
|x 1571-4853 ;
|v 2
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|u http://dx.doi.org/10.1007/0-306-47595-2
|z Full Text via HEAL-Link
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|a ZDB-2-CMS
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|a ZDB-2-BAE
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|a Chemistry and Materials Science (Springer-11644)
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