Electron Tomography Methods for Three-Dimensional Visualization of Structures in the Cell /

• Corporate Author: SpringerLink (Online service)
• Other Authors: Frank, Joachim (Editor)
• Format: Electronic eBook
• Language: English
• Published: New York, NY : Springer New York, 2006.
• Edition: Second Edition.
• Subjects: Life sciences.; Cell biology.; Microscopy.; Materials science.; Life Sciences.; Biological Microscopy.; Characterization and Evaluation of Materials.; Cell Biology.

Electron tomography has become a standard technique with applications in cell biology, structural biology, and materials science. This definitive work provides a comprehensive treatment of the mathematical background and working methods of three-dimensional reconstruction from tilt series, with spec...

Table of Contents:

• Introduction: Principles of Electron Tomography
• Sample Shrinkage and Radiation Damage of Plastic Sections
• Electron Tomography of Frozen-hydrated Sections of Cells and Tissues
• The Electron Microscope as a Structure Projector
• Cryotomography: Low-dose Automated Tomography of Frozen-hydrated Specimens
• Fiducial Marker and Hybrid Alignment Methods for Single- and Double-axis Tomography
• Markerless Alignment in Electron Tomography
• Algorithms for Three-dimensional Reconstruction From the Imperfect Projection Data Provided by Electron Microscopy
• Weighted Back-projection Methods
• Reconstruction With Orthogonal Functions
• Resolution in Electron Tomography
• Denoising of Electron Tomograms
• Segmentation of Three-dimensional Electron Tomographic Images
• Segmentation of Cell Components Using Prior Knowledge
• Motif Search in Electron Tomography
• Localization and Classification of Repetitive Structures in Electron Tomograms of Paracrystalline Assemblies.