Basic Confocal Microscopy

• Corporate Author: SpringerLink (Online service)
• Other Authors: Price, Robert L. (Editor), Jerome, W. Gray (Jay) (Editor)
• Format: Electronic eBook
• Language: English
• Published: New York, NY : Springer New York, 2011.
• Subjects: Life sciences.; Biotechnology.; Cell biology.; Microscopy.; Life Sciences.; Biological Microscopy.; Cell Biology.

Most researchers agree that biological confocal microscopy was jump-started by the confocal design first published by White and Amos in 1985 in the Journal of Cell Biology. As a result, this remains a relatively young field. Yet the use of the technique has grown phenomenally since those early effor...

Table of Contents:

• Table of Contents.- Chapter 1, Introduction and Historical Perspective.-Chapter 2, The Theory of Fluorescence.- Chapter 3, Fluorescence Microscopy.- Chapter 4, Specimen Preparation
• Chapter 5,  Labeling Considerations for Confocal Microscopy
• Chapter 6, Introduction to Digital Imaging for Confocal Microscopy
• Chapter 7, Digital Image Capture for Confocal Microscopy
• Chapter 8, Types of Confocal Instruments: Basic Principals and Advantages and Disadvantages.- Chapter 9, Setting the Operating Parameters.- Chapter 10, 3D Reconstruction of Confocal Image Data.- Chapter 11, Ethics and Resources.- Glossary  .