Lotus japonicus Handbook

• Corporate Author: SpringerLink (Online service)
• Other Authors: Márquez, Antonio J. (Editor)
• Format: Electronic eBook
• Language: English
• Published: Dordrecht : Springer Netherlands, 2005.
• Subjects: Life sciences.; Agriculture.; Plant biochemistry.; Microbiology.; Plant science.; Botany.; Plant genetics.; Plant physiology.; Life Sciences.; Plant Sciences.; Plant Genetics & Genomics.; Plant Biochemistry.; Plant Physiology.

Legumes are very important plants playing a central role in biological research. They are a key component of sustainable agricultural systems because of symbiotic nitrogen fixation and other beneficial symbiosis with mycorrhizal fungi. Studies on most of the major leguminous crops are hampered by la...

Table of Contents:

• Lotus japonicus’s a model system
• Lotus-related species and their agronomic importance
• Drought and saline stress
• Methods for studying nodule development and function
• A procedure for in vitro nodulation studies
• Arbuscular mycorrhiza
• Concurrent visualization of gusA and lacZ reporter gene expression
• Embedding root and nodule tissue in plastic (BMM)
• RNA isolation using phase extraction and LiCl precipitation
• RNA isolation using CsCl gradients
• 96-Well DNA isolation method
• Genome structure analysis
• Transcriptome analysis using cDNA arrays
• Lotus japonicus expression database
• Procedures for mass spectrometric proteome analysis
• Isolation of peribacteroid membranes for proteome analysis
• Metabolome analysis using GC-MS
• Mutagenesis
• A mutant catalogue of Lotus japonicus
• Tilling
• Ds gene-tagging
• Mapping and map-based cloning
• An in silico strategy towards the development of legume genome anchor markers using comparative sequence analysis
• Information transfer: mapping and cloning in other legumes
• Agrobacterium-mediated in vitro transformation
• Induction of hairy roots for symbiotic gene expression studies
• Transformation-regeneration procedure for Lotus japonicus
• Agrobacterium rhizogenes pRi TL-DNA integration system: a gene vector for Lotus japonicus transformation
• Vectors for reverse genetics and expression analysis
• Nitrate assimilation: Influence of nitrogen supply
• Nitrate and ammonium assimilatory enzymes
• Transgenic plants affected in nitrate assimilation
• Secondary metabolite profiling
• Phenolic Compounds: extraction and analysis
• Elicitation of isoflavan phytoalexins
• An update of work published on Lotus japonicus.