Principles and Technical Aspects of PCR Amplification

• Κύριοι συγγραφείς: Pelt-Verkuil, Elizabeth van (Συγγραφέας), Belkum, Alex van (Συγγραφέας), Hays, John P. (Συγγραφέας)
• Συγγραφή απο Οργανισμό/Αρχή: SpringerLink (Online service)
• Μορφή: Ηλεκτρονική πηγή Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Dordrecht : Springer Netherlands, 2008.
• Θέματα: Life sciences.; Biochemistry.; Cell biology.; Biology > Technique.; Life Sciences.; Life Sciences, general.; Biomedicine general.; Biochemistry, general.; Cell Biology.; Biological Techniques.

Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than 15 years ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described, with many of these adaptations and variations currently being used in clinical...

Πίνακας περιεχομένων:

• The Polymerase Chain Reaction
• A Brief Comparison Between In Vivo DNA Replication and In Vitro PCR Amplification
• The PCR in Practice
• The Different Types and Varieties of Nucleic Acid Target Molecules
• PCR Primers
• Deoxynucleotide Triphosphates and Buffer Components
• Taq and Other Thermostable DNA Polymerases
• Important Considerations for Typical, Quantitative and Real-Time PCR Protocols
• Analysis of PCR Amplification Products
• Ensuring PCR Quality – Laboratory Organisation, PCR Optimization and Controls
• Ensuring PCR Quality – Quality Criteria and Quality Assurance
• Variants and Adaptations of the Standard PCR Protocol
• In Situ PCR Amplification (ISA) – Major Considerations, Sample Processing and Applications.