Membrane Potential Imaging in the Nervous System Methods and Applications /

• Συγγραφή απο Οργανισμό/Αρχή: SpringerLink (Online service)
• Άλλοι συγγραφείς: Canepari, Marco (Επιμελητής έκδοσης), Zecevic, Dejan (Επιμελητής έκδοσης)
• Μορφή: Ηλεκτρονική πηγή Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: New York, NY : Springer New York : Imprint: Springer, 2011.
• Θέματα: Medicine.; Human physiology.; Neurosciences.; Neurobiology.; Microscopy.; Biology > Technique.; Biomedicine.; Human Physiology.; Biological Techniques.; Biological Microscopy.

Membrane Potential Imaging in the Nervous System: Methods and Applications describes optical techniques to monitor neuronal membrane potential signals. The knowledge upon which voltage-imaging technique is based was generated over several decades and the aim of this book is to provide a single compr...

Πίνακας περιεχομένων:

• Historical overview and general methods of membrane potential imaging
• Design and use of organic voltage sensitive dyes
• Imaging submillisecond membrane potential changes from individual regions of single axons, dendrites and spines
• Combined voltage and calcium imaging and signal calibration
• Use of fast-responding voltage-sensitive dyes for large-scale recording of neuronal spiking activity with single-cell resolution
• Monitoring integrated activity of individual neurons using FRET-based voltage-sensitive dyes
• Monitoring population membrane potential signals from neocortex
• Monitoring population membrane potential signals during functional development of neuronal circuits in vertebrate embryos
• Imaging the dynamics of mammalian neocortical population activity in-vivo
• Imaging the dynamics of neocortical population activity in behaving and freely moving mammals
• Monitoring membrane voltage using two-photon excitation of fluorescent voltage-sensitive dyes
• Random-access multiphoton microscopy for fast three-dimensional Imaging
• Second harmonic imaging of membrane potential
• Genetically encoded protein sensors of membrane potential.