Application of Selected Reaction Monitoring to Highly Multiplexed Targeted Quantitative Proteomics A Replacement for Western Blot Analysis /

• Κύριοι συγγραφείς: Kinter, Michael (Συγγραφέας), Kinter, Caroline S. (Συγγραφέας)
• Συγγραφή απο Οργανισμό/Αρχή: SpringerLink (Online service)
• Μορφή: Ηλεκτρονική πηγή Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: New York, NY : Springer New York : Imprint: Springer, 2013.
• Σειρά: SpringerBriefs in Systems Biology,
• Θέματα: Medicine.; Mass spectrometry.; Biochemistry.; Proteomics.; Biomedicine.; Biomedicine general.; Mass Spectrometry.; Biochemistry, general.

  A key experiment in biomedical research is monitoring the expression of different proteins in order to detect changes that occur in biological systems under different experimental conditions.  The method that is most widely used is the Western blot analysis.  While Western blot is a workhorse in l...

Πίνακας περιεχομένων:

• The use of mass spectrometry for highly selective detection
• overview of how the selected reaction monitoring experiment works
• Designing a selected reaction monitoring method for a protein
• Example analyses include: sample processing, sample analysis, data processing
• Future Directions.