Computational Methods for Three-Dimensional Microscopy Reconstruction

• Corporate Author: SpringerLink (Online service)
• Other Authors: Herman, Gabor T. (Editor), Frank, Joachim (Editor)
• Format: Electronic eBook
• Language: English
• Published: New York, NY : Springer New York : Imprint: Birkhäuser, 2014.
• Series: Applied and Numerical Harmonic Analysis,
• Subjects: Mathematics.; Bioinformatics.; Visualization.; Mathematical models.; Manifolds (Mathematics).; Complex manifolds.; Biomathematics.; Physiological, Cellular and Medical Topics.; Computational Biology/Bioinformatics.; Mathematical Modeling and Industrial Mathematics.; Manifolds and Cell Complexes (incl. Diff.Topology).

Approaches to the recovery of three-dimensional information on a biological object, which are often formulated or implemented initially in an intuitive way, are concisely described here based on physical models of the object and the image-formation process. Both three-dimensional electron microscopy...

Table of Contents:

• 1 Introduction
• 2 Interchanging geometry conventions in 3DEM: Mathematical context for the development of standards
• 3 Fully automated particle selection and verification in single-particle cryo-EM
• 4 Quantitative analysis in iterative classification schemes for cryo-EM applications
• 5 High-resolution cryo-EM structure of the Trypanosoma brucei ribosome of a case study
• 6 Computational methods for electron tomography of influenza virus
• 7 Reconstruction from microscopic projections with defocus-gradient and attenuation effects
• 8 Soft X-ray tomography imaging for biological samples
• 9 Using component trees to explore biological structures.