Atlas of Human Pluripotent Stem Cells in Culture

• Main Authors: Healy, Lyn (Author), Ruban, Ludmila (Author)
• Corporate Author: SpringerLink (Online service)
• Format: Electronic eBook
• Language: English
• Published: Boston, MA : Springer US : Imprint: Springer, 2015.
• Subjects: Life sciences.; Biochemical engineering.; Cell biology.; Stem cells.; Cell culture.; Life Sciences.; Stem Cells.; Cell Culture.; Biochemical Engineering.; Cell Biology.

This lavishly-illustrated, authoritative atlas explores the intricate art of culturing human pluripotent stem cells. Twelve chapters – containing more than 280 color illustrations – cover a variety of topics in pluripotent stem cell culturing including mouse and human fibroblasts, human embryonic st...

Table of Contents:

• 1 Before We Begin: An Introduction to the Culture of Pluripotent Cells
• 2 Mouse and Human Fibroblasts
• 3 Inactivated Mouse and Human Fibroblasts
• 4 Human Embryonic Stem Cells
• 5 Human Induced Pluripotent Stem Cells on Feeders
• 6 Pluripotent Cell Lines Grown on Different Substrates and Surfaces
• 7 Passaging Pluripotent Stem Cells
• 8 Characteristic Staining Patterns of Undifferentiated and Differentiated Pluripotent Stem Cells
• 9 Derivation of Induced Pluripotent Stem Cells
• 10 Culture Adaptation and Abnormal Cultures
• 11 Infection
• 12 Miscellaneous Cell Types and Cell Lines of Interest.