Detection and Typing Strategies for Pathogenic Escherichia coli

• Κύριοι συγγραφείς: Rivas, Lucia (Συγγραφέας), Mellor, Glen E. (Συγγραφέας), Gobius, Kari (Συγγραφέας), Fegan, Narelle (Συγγραφέας)
• Συγγραφή απο Οργανισμό/Αρχή: SpringerLink (Online service)
• Μορφή: Ηλεκτρονική πηγή Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: New York, NY : Springer New York : Imprint: Springer, 2015.
• Σειρά: SpringerBriefs in Food, Health, and Nutrition,
• Θέματα: Chemistry.; Microbiology.; Food > Biotechnology.; Bacteriology.; Food Science.; Applied Microbiology.

This Brief will review the methods that are currently available for the detection, isolation, and typing of pathogenic E. coli with a particular focus on foodborne diseases caused by the Shiga toxigenic E. coli group, which have been implicated in a number of significant outbreaks in recent years. P...

Πίνακας περιεχομένων:

• Chapter 1 Introduction to Pathogenic Escherichia coli
• 1.1 Pathotypes of Escherichia coli causing diarrhoeal diseases
• 1.2 Escherichia coli
• 1.3 Pathogenic types of E. coli
• 1.4 Shiga toxin-producing and Enterohemorrhagic E. coli
• 1.4.1    Virulence determinants of Shiga-toxin producing E. coli
• 1.4.2 Shiga-toxin producing E. coli-mediated disease
• 1.4.3 Epidemiology of Shiga toxin-producing E. coli
• 1.4.4    Ecology of Shiga-toxin producing E. coli
• 1.4.5 Transmission of Shiga-toxin producing E. coli
• 1.4.6 Shiga-toxin producing E. coli occurrence in foods
• 1.5 Enterotoxigenic E. coli
• 1.6 Enteropathogenic E. coli
• 1.7 Enteroinvasive E. coli
• 1.8 Enteroaggregative and Diffusely Adherent E. coli..-1.9 References
• Chapter 2 Isolation and detection of pathogenic Escherichia coli in foods
• 2.1 Introduction
• 2.2 General method of isolation for E. coli
• 2.3 Shiga toxin-producing E. coli
• 2.3.1 Culture and isolation of Shiga toxin-producing E. coli
• 2.3.2    Molecular detection of Shiga toxin-producing E. coli
• 2.4 Enumeration of Shiga toxin-producing E.coli
• 2.5 Immunological detection methods for pathogenic E. coli
• 2.6 Cell culture assays used for pathogenic E. coli
• 2.6.1 Cell cytotoxicity assay for Shiga toxin-producing E. coli
• 2.6.2    Cell adherence assays
• 2.7 Enteroinvasive E. coli
• 2.8 Enterotoxigenic E. coli
• 2.9 Enteropathogenic E. coli
• 2.10     Enteroaggregative E. coli and Diffusely Adherent E. coli
• 2.11 References
• Chapter 3 Typing and Subtyping methods for pathogenic Escherichia coli
• 3.1 Introduction
• 3.2 Biochemical profiling
• 3.3 Serotyping
• 3.4 Phage typing
• 3.5 Multilocus enzyme electrophoresis
• 3.6 Multilocus sequence typing
• 3.7 Pulsed-field gel electrophoresis
• 3.8 Multiple-locus variable number tandem repeat analysis
• 3.9 Repetitive element palindromic polymerase chain reaction
• 3.10 Random amplified polymorphic DNA
• 3.11 Shiga toxin subtypes and bacteriophage insertion sites
• 3.12 Lineage specific polymorphism analysis
• 3.13 Whole genome sequencing
• 3.14
• References
• Chapter 4 Emerging and future trends and technologies for the detection and typing of Escherichia coli
• 4.1 Future trends in foodborne illness and E. coli
• 4.2 Future trends in the detection and typing of pathogens
• 4.2.1 Microarrays
• 4.2.2 Matrix Assisted Laser-Desorption Ionization-Time-Of-Flight Mass Spectrometry
• 4.2.3 Nanotechnology
• 4.2.4 Next-generating sequencing of the whole bacterial genome
• 4.3 References.