Atlas of Human Pluripotent Stem Cells Derivation and Culturing /

• Corporate Author: SpringerLink (Online service)
• Other Authors: Amit, Michal (Editor), Itskovitz-Eldor, Joseph (Editor)
• Format: Electronic eBook
• Language: English
• Published: Totowa, NJ : Humana Press : Imprint: Humana Press, 2012.
• Series: Stem Cell Biology and Regenerative Medicine
• Subjects: Life sciences.; Biotechnology.; Cell biology.; Stem cells.; Microscopy.; Life Sciences.; Biological Microscopy.; Stem Cells.; Cell Biology.

Human pluripotent stem cells, including human embryonic stem cells and induced pluripotent stem cells, are a key focus of current biomedical research. The emergence of state of the art culturing techniques is promoting the realization of the full potential of pluripotent stem cells in basic and tran...

Table of Contents:

• Methods for the Derivation of Human Embryonic Stem Cell Lines
• Morphology of Human Embryonic and Induced Pluripotent Stem Cell Colonies Cultured with Feeders
• Morphology of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells Cultured in Feeder-Layer-Free Conditions
• Morphology of Undifferentiated Human Embryonic and Induced Stem Cells Grown in Suspension and in Dynamic Cultures
• Differentiation of Pluripotent Stems Cells in vitro: Embryoid Bodies
• Differentiation of Pluripotet Stems Cells in vivo: Teratoma Formation
• Immunostaining
• Karyotype Analysis of Human Embryonic Stem Cell and Induced Pluripotent Stem Cell Lines
• Method for the Derivation of Induced Pluripotent Stem Cells from Human Hair Follicle Keratinocytes.