Fluorescent Methods for Molecular Motors

• Συγγραφή απο Οργανισμό/Αρχή: SpringerLink (Online service)
• Άλλοι συγγραφείς: Toseland, Christopher P. (Επιμελητής έκδοσης), Fili, Natalia (Επιμελητής έκδοσης)
• Μορφή: Ηλεκτρονική πηγή Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Basel : Springer Basel : Imprint: Springer, 2014.
• Σειρά: Experientia Supplementum, 105
• Θέματα: Life sciences.; Proteins.; Cell biology.; Biophysics.; Life Sciences.; Protein Science.; Cell Biology.; Single Molecule Studies, Molecular Motors.

This book focuses on the application of fluorescence to study motor proteins (myosins, kinesins, DNA helicases and RNA polymerases). It is intended for a large community of biochemists, biophysicists and cell biologists who study a diverse collection of motor proteins. It can be used by researchers...

Πίνακας περιεχομένων:

• Fluorescence and Labelling: How to choose and what to do
• Fluorescent biosensors: design and application to motor proteins
• Rapid Reaction Kinetic Techniques
• Fluorescence to study the ATPase mechanism of Motor Proteins
• Use of pyrene labelled actin to probe actin myosin interactions; kinetic and equilibrium studies
• Fluorescent methods to study transcription initiation and transition into elongation
• Single-molecule and single-particle imaging of molecular motors in vitro and in vivo
• Fluorescence methods in the investigation of the DEAD-box helicase mechanism
• Use of Fluorescent Techniques to Study the In Vitro Movement of Myosins
• Fluorescence Tracking of Motor proteins in vitro
• Measuring Transport of Motor Cargos
• Measuring two at the same time: Combining Magnetic Tweezers with Single-Molecule FRET
• Using fluorescence to study actomyosin in yeasts.