How Free Cationic Polymer Chains Promote Gene Transfection

• Main Author: Yanan, Yue (Author)
• Corporate Author: SpringerLink (Online service)
• Format: Electronic eBook
• Language: English
• Published: Heidelberg : Springer International Publishing : Imprint: Springer, 2013.
• Series: Springer Theses, Recognizing Outstanding Ph.D. Research,
• Subjects: Chemistry.; Organic chemistry.; Polymers.; Nucleic acids.; Polymer Sciences.; Organic Chemistry.; Nucleic Acid Chemistry.

In this PhD thesis, Yue Yanan addresses a long-overlooked and critical question in the development of non-viral vectors for gene delivery. The author determines that those uncomplexed and cationic polymer chains free in the solution mixture of polymer and DNA facilitate and promote gene transfection...

Table of Contents:

• Introduction and background
• Revisiting complexation between DNA and polyethylenimine: the effect of uncomplexed chains free in the solution mixture on gene transfection
• Revisiting complexation between DNA and polyethylenimine: the effect of length of free polycationic chains on gene transfection
• Quantitative comparison of endocytosis and intracellular trafficking of DNA/polymer complexes in the absence/presence of free polycationic chains.