Plant Microtechniques and Protocols

• Συγγραφή απο Οργανισμό/Αρχή: SpringerLink (Online service)
• Άλλοι συγγραφείς: Yeung, Edward Chee Tak (Επιμελητής έκδοσης), Stasolla, Claudio (Επιμελητής έκδοσης), Sumner, Michael John (Επιμελητής έκδοσης), Huang, Bing Quan (Επιμελητής έκδοσης)
• Μορφή: Ηλεκτρονική πηγή Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Cham : Springer International Publishing : Imprint: Springer, 2015.
• Έκδοση: 1st ed. 2015.
• Θέματα: Life sciences.; Plant anatomy.; Plant development.; Plant breeding.; Life Sciences.; Plant Breeding/Biotechnology.; Plant Anatomy/Development.; Plant Systematics/Taxonomy/Biogeography.

This work gathers common plant microtechniques and updates their procedures using a simple and fully understandable approach.  The text serves as a handy resource for scientists familiar with the protocols and as a guide for the novice, especially students just beginning to learn about various struc...

Πίνακας περιεχομένων:

• A Guide to the Study of Plant Structure with Emphasis on Living Specimens
• Chemical and Physical Fixation of Cells and Tissues: an Overview
• Paraffin and Polyester Waxes
• The Glycol Methacrylate Embedding Resins – Technovit 7100 and 8100
• Epoxy Resins for Light and Transmission Electron Microscopy
• LR White Acrylic Resin
• High Pressure Freezing and Freeze Substitution of In vivo and In vitro Cultured Plant Samples
• Three-Dimensional Imaging for Electron Microscopy of Plastic-embedded Plant Specimens
• Fluorescent Staining of Living Plant Cells
• Improved Methods for Clearing and Staining of Plant Samples
• Whole Mount Immunofluorescence Staining of Plant Cells and Tissues
• Protoplast Isolation and Staining
• Guiding Principles for Live Cell Imaging of Plants using Confocal Microscopy.-Immunogold Labeling for Electron Microscopy: Strategy and Problem Solving
• Abscisic Acid Immunostaining
• Plant Chromosome Preparations and Staining for Light Microscopic Studies
• Chromosome techniques and FISH
• Detection of S-phase of Cell Division Cycle in Plant Cells and Tissues by using 5-ethynyl-2’-deoxyuridine (EdU)
• Staining Methods for Programmed Cell Death
• Laser Microdissection of Plant Tissues
• RNA In situ Hybridization
• Microscopic Examination of Wood: Sample Preparation and Techniques for Light Microscopy
• Collecting and Processing Wood Microcores for Monitoring Xylogenesis
• Three-dimensional Imaging of Cambium and Secondary Xylem Cells by Confocal Laser Scanning Microscopy
• Collection of Plant Remains from Archaeological Contexts
• Archaeological Wood Preparation
• Archaeopalynological Preparation Techniques
• Phytoliths: Preparation and Archaeological Extraction
• Starch Granules: Preparation and Archaeological Extraction
• Plant Collection, Identification, and Herbarium Procedures
• Index.