Using Mass Spectrometry for Biochemical Studies on Enzymatic Domains from Polyketide Synthases

• Κύριος συγγραφέας: Jenner, Matthew (Συγγραφέας)
• Συγγραφή απο Οργανισμό/Αρχή: SpringerLink (Online service)
• Μορφή: Ηλεκτρονική πηγή Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Cham : Springer International Publishing : Imprint: Springer, 2016.
• Σειρά: Springer Theses, Recognizing Outstanding Ph.D. Research,
• Θέματα: Chemistry.; Mass spectrometry.; Biochemical engineering.; Medical biochemistry.; Enzymology.; Mass Spectrometry.; Biochemical Engineering.; Medical Biochemistry.

This thesis reports studies on the substrate specificity of crucial ketosynthase (KS) domains from trans-AT Polyketide Synthases (PKSs). Using a combination of electrospray ionisation-mass spectrometry (ESI-MS) and simple N-acetyl cysteamine (SNAC) substrate mimics, Matthew Jenner has successfully s...

Πίνακας περιεχομένων:

• Introduction
• Materials and Methods
• Substrate Specificity of Ketosynthase Domains Part I: β-Branched Acyl Chains
• Substrate Specificity of Ketosynthase Domains Part II: Amino Acid-Containing Acyl Chains
• Synthesis of Acyl-Acyl Carrier Proteins and their use in Studying Polyketide Synthase Enzymology
• Substrate Specificity of Ketosynthase Domains Part III: Elongation-Based Substrate Specificity.