Using Mass Spectrometry for Biochemical Studies on Enzymatic Domains from Polyketide Synthases

• Main Author: Jenner, Matthew (Author)
• Corporate Author: SpringerLink (Online service)
• Format: Electronic eBook
• Language: English
• Published: Cham : Springer International Publishing : Imprint: Springer, 2016.
• Series: Springer Theses, Recognizing Outstanding Ph.D. Research,
• Subjects: Chemistry.; Mass spectrometry.; Biochemical engineering.; Medical biochemistry.; Enzymology.; Mass Spectrometry.; Biochemical Engineering.; Medical Biochemistry.

This thesis reports studies on the substrate specificity of crucial ketosynthase (KS) domains from trans-AT Polyketide Synthases (PKSs). Using a combination of electrospray ionisation-mass spectrometry (ESI-MS) and simple N-acetyl cysteamine (SNAC) substrate mimics, Matthew Jenner has successfully s...

Table of Contents:

• Introduction
• Materials and Methods
• Substrate Specificity of Ketosynthase Domains Part I: β-Branched Acyl Chains
• Substrate Specificity of Ketosynthase Domains Part II: Amino Acid-Containing Acyl Chains
• Synthesis of Acyl-Acyl Carrier Proteins and their use in Studying Polyketide Synthase Enzymology
• Substrate Specificity of Ketosynthase Domains Part III: Elongation-Based Substrate Specificity.