Isolation and Molecular Characterization of Circulating Tumor Cells

• Corporate Author: SpringerLink (Online service)
• Other Authors: Magbanua, Mark Jesus M. (Editor), Park, John W. (Editor)
• Format: Electronic eBook
• Language: English
• Published: Cham : Springer International Publishing : Imprint: Springer, 2017.
• Series: Advances in Experimental Medicine and Biology, 994
• Subjects: Medicine.; Cancer research.; Biomedicine.; Cancer Research.

The past decade has seen remarkable progress in elucidating the clinical relevance of circulating tumor cells (CTCs). Evidence from numerous clinical studies has unequivocally demonstrated the importance of CTCs as harbingers of poor outcome. Despite these advances, the molecular nature of CTCs rema...

Table of Contents:

• Preface
• Circulating Tumor Cells as Cancer Biomarkers
• Strategies for Isolation and Molecular Profiling of Circulating Tumor Cells
• Aptamer-Based Methods for Detection of Circulating Tumor Cells and Their Potential for Personalized Diagnostics
• Development of a Protocol for Single-Cell Analysis of Circulating Tumor Cells in Patients with Solid Tumors
• Flow Cytometric Methods for Circulating Tumor Cell Isolation and Molecular Analysis
• Enrichment and Detection of Circulating Tumor Cells and Other Rare Cell Populations by Microfluidic Filtration
• Detection and Enumeration of Circulating Tumor Cells with Invasive Phenotype
• Molecular Profiling and Significance of Circulating Tumor Cell Based Genetic Signatures
• Detection of Gene-Rearrangements in Circulating Tumor Cells: Examples of ALK-, ROS1-, RET-Rearrangements in Non-Small-Cell Lung Cancer and ERG-Rearrangements in Prostate Cancer
• Enrichment, Isolation, and Molecular Characterization of EpCAM-Negative Circulating Tumor Cells
• Expression of Epithelial Mesenchymal Transition and Cancer Stem Cell Markers in Circulating Tumor Cells.