Decoding the Antibody Repertoire High Throughput Sequencing of Multiple Transcripts from Single B Cells /

• Κύριος συγγραφέας: DeKosky, Brandon (Συγγραφέας)
• Συγγραφή απο Οργανισμό/Αρχή: SpringerLink (Online service)
• Μορφή: Ηλεκτρονική πηγή Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Cham : Springer International Publishing : Imprint: Springer, 2017.
• Σειρά: Springer Theses, Recognizing Outstanding Ph.D. Research,
• Θέματα: Medicine.; Human genetics.; Antibodies.; Genetic engineering.; Biochemistry.; Cell biology.; Biomedicine.; Genetic Engineering.; Human Genetics.; Cell Biology.; Biochemistry, general.

This thesis outlines the development of the very first technology for high-throughput analysis of paired heavy and light-chain antibody sequences, opening the door for the discovery of new antibodies and the investigation of adaptive immune responses to vaccines and diseases. By designing two new te...

Πίνακας περιεχομένων:

• Background
• High-throughput Sequencing of the Paired Human Immunoglobulin Heavy and Light Chain Repertoire
• In-Depth Determination and Analysis of the Human Paired Heavy and Light Chain Antibody Repertoire
• Paired VH:VL Analysis of Naïve B Cell Repertoires and Comparison to Antigen-Experienced B Cell Repertoires in Healthy Human Donors
• Conclusions and Future Perspectives
• Appendices.