Decoding the Antibody Repertoire High Throughput Sequencing of Multiple Transcripts from Single B Cells /

• Main Author: DeKosky, Brandon (Author)
• Corporate Author: SpringerLink (Online service)
• Format: Electronic eBook
• Language: English
• Published: Cham : Springer International Publishing : Imprint: Springer, 2017.
• Series: Springer Theses, Recognizing Outstanding Ph.D. Research,
• Subjects: Medicine.; Human genetics.; Antibodies.; Genetic engineering.; Biochemistry.; Cell biology.; Biomedicine.; Genetic Engineering.; Human Genetics.; Cell Biology.; Biochemistry, general.

This thesis outlines the development of the very first technology for high-throughput analysis of paired heavy and light-chain antibody sequences, opening the door for the discovery of new antibodies and the investigation of adaptive immune responses to vaccines and diseases. By designing two new te...

Table of Contents:

• Background
• High-throughput Sequencing of the Paired Human Immunoglobulin Heavy and Light Chain Repertoire
• In-Depth Determination and Analysis of the Human Paired Heavy and Light Chain Antibody Repertoire
• Paired VH:VL Analysis of Naïve B Cell Repertoires and Comparison to Antigen-Experienced B Cell Repertoires in Healthy Human Donors
• Conclusions and Future Perspectives
• Appendices.