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03643nam a22005415i 4500 |
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978-3-319-67358-5 |
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DE-He213 |
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20171027141002.0 |
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cr nn 008mamaa |
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171027s2017 gw | s |||| 0|eng d |
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|a 9783319673585
|9 978-3-319-67358-5
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|a 10.1007/978-3-319-67358-5
|2 doi
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|a Multi-Parametric Live Cell Microscopy of 3D Tissue Models
|h [electronic resource] /
|c edited by Ruslan I. Dmitriev.
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| 264 |
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1 |
|a Cham :
|b Springer International Publishing :
|b Imprint: Springer,
|c 2017.
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| 300 |
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|a XII, 172 p. 53 illus., 39 illus. in color.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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| 347 |
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|a text file
|b PDF
|2 rda
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1 |
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|a Advances in Experimental Medicine and Biology,
|x 0065-2598 ;
|v 1035
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| 505 |
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|a Current state-of-the-art 3D tissue models and their compatibility with live cell imaging -- Simultaneous Phosphorescence and Fluorescence Lifetime Imaging by Multi-Dimensional TCSPC and Multi-Pulse Excitation -- Quantitative live cell FLIM imaging in three dimensions -- Three-dimensional tissue models and available probes for multi-parametric live cell microscopy: a brief overview -- Fabrication and handling of 3D scaffolds based on polymers and decellularized tissues -- Multi-parametric imaging of hypoxia and cell cycle in intestinal organoid culture -- Imaging of intracellular pH in tumor spheroids using genetically encoded sensor SypHer2 -- Application of fluorescence lifetime imaging (FLIM) to measure intracellular environments in a single cell -- Quantitative imaging of Ca2+ by 3D-FLIM in live tissues -- Live cell imaging of viscosity in 3D tumour cell models -- Live imaging of cell invasion using a multicellular spheroid model and lightsheet microscopy -- Raman imaging microscopy for quantitative analysis of biological samples.
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| 520 |
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|a This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.
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| 650 |
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|a Life sciences.
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| 650 |
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|a Biomedical engineering.
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| 650 |
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|a Regenerative medicine.
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| 650 |
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|a Tissue engineering.
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| 650 |
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|a Microscopy.
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| 650 |
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|a Spectroscopy.
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| 650 |
1 |
4 |
|a Life Sciences.
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| 650 |
2 |
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|a Regenerative Medicine/Tissue Engineering.
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| 650 |
2 |
4 |
|a Biological Microscopy.
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| 650 |
2 |
4 |
|a Biomedical Engineering/Biotechnology.
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| 650 |
2 |
4 |
|a Biomedical Engineering.
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| 650 |
2 |
4 |
|a Spectroscopy and Microscopy.
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| 700 |
1 |
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|a Dmitriev, Ruslan I.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 776 |
0 |
8 |
|i Printed edition:
|z 9783319673578
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| 830 |
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0 |
|a Advances in Experimental Medicine and Biology,
|x 0065-2598 ;
|v 1035
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| 856 |
4 |
0 |
|u http://dx.doi.org/10.1007/978-3-319-67358-5
|z Full Text via HEAL-Link
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| 912 |
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|a ZDB-2-SBL
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| 950 |
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|a Biomedical and Life Sciences (Springer-11642)
|
