Multi-Parametric Live Cell Microscopy of 3D Tissue Models

• Corporate Author: SpringerLink (Online service)
• Other Authors: Dmitriev, Ruslan I. (Editor)
• Format: Electronic eBook
• Language: English
• Published: Cham : Springer International Publishing : Imprint: Springer, 2017.
• Series: Advances in Experimental Medicine and Biology, 1035
• Subjects: Life sciences.; Biomedical engineering.; Regenerative medicine.; Tissue engineering.; Microscopy.; Spectroscopy.; Life Sciences.; Regenerative Medicine/Tissue Engineering.; Biological Microscopy.; Biomedical Engineering/Biotechnology.; Biomedical Engineering.; Spectroscopy and Microscopy.

This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence...

Table of Contents:

• Current state-of-the-art 3D tissue models and their compatibility with live cell imaging
• Simultaneous Phosphorescence and Fluorescence Lifetime Imaging by Multi-Dimensional TCSPC and Multi-Pulse Excitation
• Quantitative live cell FLIM imaging in three dimensions
• Three-dimensional tissue models and available probes for multi-parametric live cell microscopy: a brief overview
• Fabrication and handling of 3D scaffolds based on polymers and decellularized tissues
• Multi-parametric imaging of hypoxia and cell cycle in intestinal organoid culture
• Imaging of intracellular pH in tumor spheroids using genetically encoded sensor SypHer2
• Application of fluorescence lifetime imaging (FLIM) to measure intracellular environments in a single cell
• Quantitative imaging of Ca2+ by 3D-FLIM in live tissues
• Live cell imaging of viscosity in 3D tumour cell models
• Live imaging of cell invasion using a multicellular spheroid model and lightsheet microscopy
• Raman imaging microscopy for quantitative analysis of biological samples.