Flow Cytometry Basics for the Non-Expert

• Main Authors: Goetz, Christine (Author, http://id.loc.gov/vocabulary/relators/aut), Hammerbeck, Christopher (http://id.loc.gov/vocabulary/relators/aut), Bonnevier, Jody (http://id.loc.gov/vocabulary/relators/aut)
• Corporate Author: SpringerLink (Online service)
• Format: Electronic eBook
• Language: English
• Published: Cham : Springer International Publishing : Imprint: Springer, 2018.
• Edition: 1st ed. 2018.
• Series: Techniques in Life Science and Biomedicine for the Non-Expert,
• Subjects: Antibodies.; Laboratory medicine.; Laboratory Medicine.

Flow cytometry allows for the measurement of different aspects of a cell or particles in real time. Applications include quantifying various types of T cells to determine the presence of various immune disorders, such as whether HIV has progressed to AIDS, studying the dynamics of immune signaling i...

Table of Contents:

• 1. Flow Cytometry: Definition, History, and Uses in Biotechnology
• 2. Physics of a Flow Cytometer
• 3. The Language of Flow Cytometry and Experimental Setup
• 4. Fluorochrome Choices for Flow Cytometry
• 5. Compensation
• 6. Primary and Seconardy Antibodies and Their Use in Flow Cytometry
• 7. Experimental Considerations with Data Sets as Examples
• 8. Cell Enrichment
• 9. Surface and Intracellular Staining Protocols for Flow Cytometry
• 10. Troubleshooting.