Mammalian and Avian Transgenesis — New Approaches

• Συγγραφή απο Οργανισμό/Αρχή: SpringerLink (Online service)
• Άλλοι συγγραφείς: Pease, Shirley (Επιμελητής έκδοσης), Lois, Carlos (Επιμελητής έκδοσης)
• Μορφή: Ηλεκτρονική πηγή Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Berlin, Heidelberg : Springer Berlin Heidelberg, 2006.
• Σειρά: Principles and Practice
• Θέματα: Life sciences.; Molecular biology.; Cell biology.; Developmental biology.; Animal genetics.; Life Sciences.; Animal Genetics and Genomics.; Cell Biology.; Developmental Biology.; Molecular Medicine.

 Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds. The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis. 

 Generation of Transgenic Animals Using Lentiviral Vectors -- Intracytoplasmic Sperm Injection (ICSI) in the Mouse -- Generation of Embryonic Stem (ES) Cell-Derived Embryos and Mice by Tetraploid-Embryo Complementation -- Cloning The Laboratory Mouse by Nuclear Transfer -- Large Insert Transgenesis -- Regional and Temporal Control of Genetic Manipulation in the Mouse -- High Resolution Gene Expression Analysis in Mice Using Genetically Inserted Reporter Genes -- Nuclear Transfer in the Cow -- Production of Transgenic Pigs by DNA Microinjection -- Production of Transgenic Birds Using Lentiviral Vectors -- Ancillary Techniques.