Mammalian and Avian Transgenesis — New Approaches

• Corporate Author: SpringerLink (Online service)
• Other Authors: Pease, Shirley (Editor), Lois, Carlos (Editor)
• Format: Electronic eBook
• Language: English
• Published: Berlin, Heidelberg : Springer Berlin Heidelberg, 2006.
• Series: Principles and Practice
• Subjects: Life sciences.; Molecular biology.; Cell biology.; Developmental biology.; Animal genetics.; Life Sciences.; Animal Genetics and Genomics.; Cell Biology.; Developmental Biology.; Molecular Medicine.

Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds. The manual provides detailed, step-by-step prot...

Table of Contents:

• Generation of Transgenic Animals Using Lentiviral Vectors
• Intracytoplasmic Sperm Injection (ICSI) in the Mouse
• Generation of Embryonic Stem (ES) Cell-Derived Embryos and Mice by Tetraploid-Embryo Complementation
• Cloning The Laboratory Mouse by Nuclear Transfer
• Large Insert Transgenesis
• Regional and Temporal Control of Genetic Manipulation in the Mouse
• High Resolution Gene Expression Analysis in Mice Using Genetically Inserted Reporter Genes
• Nuclear Transfer in the Cow
• Production of Transgenic Pigs by DNA Microinjection
• Production of Transgenic Birds Using Lentiviral Vectors
• Ancillary Techniques.