Guidelines for Molecular Analysis in Archive Tissues

• Corporate Author: SpringerLink (Online service)
• Other Authors: Stanta, Giorgio (Editor)
• Format: Electronic eBook
• Language: English
• Published: Berlin, Heidelberg : Springer Berlin Heidelberg, 2011.
• Subjects: Medicine.; Molecular biology.; Oncology.; Pathology.; Medicine & Public Health.; Molecular Medicine.

A huge amount of fixed and paraffin-embedded tissue is stored in every hospital. This is very precious material that can be used for translational research and for diagnostics. The molecular methods employed for analysis of these tissues are similar to the usual molecular biology and proteomics meth...

Table of Contents:

• Introduction
• Archive Tissues
• Pre-Analytical Treatment of Tissues
• Dissection of Tissue Components
• Extraction, Purification, Quantification and Quality Assessment of Nucleic Acids
• Reverse Transcription (RT)
• Nucleic Acid Amplification
• DNA Sequencing
• Microsatellite Analysis
• Methylation Analysis
• Analysis of Copy Number Variations
• Microarrays
• Protein Extraction in Archive Tissues
• Protein Electrophoretic Separation
• Protein Immunodetection
• Mass Spectrometry
• Elements of Good Laboratory Practice.
• DNA Sequencing
• Microsatellite Analysis
• Methylation Analysis
• Analysis of Copy Number Variations
• Microarrays
• Protein Extraction in Archive Tissues
• Protein Electrophoretic Separation
• Protein Immunodetection
• Mass Spectrometry
• Elements of Good Laboratory Practice.