Guidelines for Molecular Analysis in Archive Tissues

• Συγγραφή απο Οργανισμό/Αρχή: SpringerLink (Online service)
• Άλλοι συγγραφείς: Stanta, Giorgio (Επιμελητής έκδοσης)
• Μορφή: Ηλεκτρονική πηγή Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Berlin, Heidelberg : Springer Berlin Heidelberg, 2011.
• Θέματα: Medicine.; Molecular biology.; Oncology.; Pathology.; Medicine & Public Health.; Molecular Medicine.

A huge amount of fixed and paraffin-embedded tissue is stored in every hospital. This is very precious material that can be used for translational research and for diagnostics. The molecular methods employed for analysis of these tissues are similar to the usual molecular biology and proteomics meth...

Πίνακας περιεχομένων:

• Introduction
• Archive Tissues
• Pre-Analytical Treatment of Tissues
• Dissection of Tissue Components
• Extraction, Purification, Quantification and Quality Assessment of Nucleic Acids
• Reverse Transcription (RT)
• Nucleic Acid Amplification
• DNA Sequencing
• Microsatellite Analysis
• Methylation Analysis
• Analysis of Copy Number Variations
• Microarrays
• Protein Extraction in Archive Tissues
• Protein Electrophoretic Separation
• Protein Immunodetection
• Mass Spectrometry
• Elements of Good Laboratory Practice.
• DNA Sequencing
• Microsatellite Analysis
• Methylation Analysis
• Analysis of Copy Number Variations
• Microarrays
• Protein Extraction in Archive Tissues
• Protein Electrophoretic Separation
• Protein Immunodetection
• Mass Spectrometry
• Elements of Good Laboratory Practice.