Gigaseal Formation in Patch Clamping With Applications of Nanotechnology /

This book presents an investigation of gigaseal formation using micro/nanotechnology. The aims of the book are twofold. First, it explains the mechanisms of gigaseal formation using the latest discoveries. Second, it provides practical techniques for frequent formation of high resistance seals. The...

Πλήρης περιγραφή

Λεπτομέρειες βιβλιογραφικής εγγραφής
Κύριοι συγγραφείς: Malboubi, Majid (Συγγραφέας), Jiang, Kyle (Συγγραφέας)
Συγγραφή απο Οργανισμό/Αρχή: SpringerLink (Online service)
Μορφή: Ηλεκτρονική πηγή Ηλ. βιβλίο
Γλώσσα:English
Έκδοση: Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 2014.
Σειρά:SpringerBriefs in Applied Sciences and Technology,
Θέματα:
Διαθέσιμο Online:Full Text via HEAL-Link
LEADER 03075nam a22004695i 4500
001 978-3-642-39128-6
003 DE-He213
005 20151103124530.0
007 cr nn 008mamaa
008 130724s2014 gw | s |||| 0|eng d
020 |a 9783642391286  |9 978-3-642-39128-6 
024 7 |a 10.1007/978-3-642-39128-6  |2 doi 
040 |d GrThAP 
050 4 |a T174.7 
072 7 |a TDPB  |2 bicssc 
072 7 |a TEC027000  |2 bisacsh 
082 0 4 |a 620.5  |2 23 
100 1 |a Malboubi, Majid.  |e author. 
245 1 0 |a Gigaseal Formation in Patch Clamping  |h [electronic resource] :  |b With Applications of Nanotechnology /  |c by Majid Malboubi, Kyle Jiang. 
264 1 |a Berlin, Heidelberg :  |b Springer Berlin Heidelberg :  |b Imprint: Springer,  |c 2014. 
300 |a XI, 94 p. 81 illus., 65 illus. in color.  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
347 |a text file  |b PDF  |2 rda 
490 1 |a SpringerBriefs in Applied Sciences and Technology,  |x 2191-530X 
505 0 |a Chapter 1 Introduction -- Chapter 2 Development of patch clamping -- Chapter 3 Gigaseal formation -- Chapter 4 Effect of roughness on gigaseal formation -- Chapter 5 Effect of hydrophilicIty on gigaseal formation -- Chapter 6 Effect of tip size on gigaseal formation -- Chapter 7 Study of glass micropipettes from tip formation to characterization -- References. 
520 |a This book presents an investigation of gigaseal formation using micro/nanotechnology. The aims of the book are twofold. First, it explains the mechanisms of gigaseal formation using the latest discoveries. Second, it provides practical techniques for frequent formation of high resistance seals. The formation of a high-resistance electrical seal, also known as a gigaseal, between a cell membrane and a glass micropipette tip is essential in patch-clamp experiments. Even though four decades have passed since the introduction of the patch-clamping technique by Neher and Sakmann, gigaseal formation remains an obstacle in developing the high-throughput ion channel screening systems required by the pharmaceutical industry. Here the authors share their latest methods for achieving gigaseal formation and describe techniques that are highly desirable at both research and industrial levels. Nanotechnology has been found to be a powerful tool for studying and modifying glass micropipettes and in tackling the problem of gigaseal formation. 
650 0 |a Engineering. 
650 0 |a Nanotechnology. 
650 0 |a Biomedical engineering. 
650 1 4 |a Engineering. 
650 2 4 |a Nanotechnology and Microengineering. 
650 2 4 |a Biomedical Engineering. 
700 1 |a Jiang, Kyle.  |e author. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
776 0 8 |i Printed edition:  |z 9783642391279 
830 0 |a SpringerBriefs in Applied Sciences and Technology,  |x 2191-530X 
856 4 0 |u http://dx.doi.org/10.1007/978-3-642-39128-6  |z Full Text via HEAL-Link 
912 |a ZDB-2-ENG 
950 |a Engineering (Springer-11647)