Applications of Aminoacylation Ribozymes That Recognize the 3′-end of tRNA

• Main Author: Terasaka, Naohiro (Author)
• Corporate Author: SpringerLink (Online service)
• Format: Electronic eBook
• Language: English
• Published: Tokyo : Springer Japan : Imprint: Springer, 2017.
• Series: Springer Theses, Recognizing Outstanding Ph.D. Research,
• Subjects: Chemistry.; Biotechnology.; Bioorganic chemistry.; Catalysis.; Nucleic acids.; Bioorganic Chemistry.; Nucleic Acid Chemistry.

In this thesis, applications of aminoacylation ribozymes named flexizymes are described. Flexizymes have the following unique characteristics: (i) substrate RNA is recognized by two consecutive base pairs between the 3'-end of substrate RNA and the 3'-end of the flexizyme; (ii) these base...

Table of Contents:

• General introduction
• Discovery of human microRNA precursor binding to folic acid by small RNA transcriptomic SELEX
• Orthogonal ribosome-tRNAs pair by engineering of peptidyl transferase center
• General conclusion.